Evidence map›Paper›PMID 32529359›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2020

Isolation and Analysis of Mitochondrial Fission Enzyme DNM1 from Saccharomyces cerevisiae.

Nolan W Kennedy, Lora K Picton, R Blake Hill

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.4field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Nolan W KennedyDepartment of Biochemistry, Medical College of Wisconsin, Milwaukee, WI, USA.
Lora K PictonDepartment of Biochemistry, Medical College of Wisconsin, Milwaukee, WI, USA.
R Blake HillDepartment of Biochemistry, Medical College of Wisconsin, Milwaukee, WI, USA. rbhill@mcw.edu.
Medical College of Wisconsin · US

Funding

Structural Biology of Mitochondrial FissionR01GM067180 · NIGMS · MEDICAL COLLEGE OF WISCONSIN · PI HILL, R BLAKE · 2004 to 2022
$6.0M
NIGMS NIH HHS R01 GM067180
6 · The paper itself

Abstract

Mitochondrial fission, an essential process for mitochondrial and cellular homeostasis, is accomplished by evolutionarily conserved members of the dynamin superfamily of large GTPases. These enzymes couple the hydrolysis of guanosine triphosphate to the mechanical work of membrane remodeling that ultimately leads to membrane scission. The importance of mitochondrial dynamins is exemplified by mutations in the human family member that causes neonatal lethality. In this chapter, we describe the subcloning, purification, and preliminary characterization of the budding yeast mitochondrial dynamin, DNM1, from Saccharomyces cerevisiae, which is the first mitochondrial dynamin isolated from native sources. The yeast-purified enzyme exhibits assembly-stimulated hydrolysis of GTP similar to other fission dynamins, but differs from the enzyme isolated from non-native sources.

Indexed as

Mitochondrial DynamicsChromatographyCloning, MolecularGTP PhosphohydrolasesGuanosine TriphosphateHydrolysisMitochondrial ProteinsSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsDNM1 protein, S cerevisiaeGTP PhosphohydrolasesGuanosine TriphosphateMitochondrial ProteinsSaccharomyces cerevisiae ProteinsDrp1DynaminMembrane scissionMitochondrial dynamicsMitochondrial fission

Identifiers

PMID32529359
PMCPMC8040746
OpenAlexW3035517310

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.