ArticleAnalytical and bioanalytical chemistry2020
Simultaneous analysis of multiple oligonucleotides by temperature-responsive chromatography using a poly(N-isopropylacrylamide)-based stationary phase.
Article in Analytical and bioanalytical chemistry, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 23 citations in OpenAlex.
- Pore-Based RNA Evaluation for Control of Integrity, Sequence, and Errors - Quality Control (PRECISE-QC).bioRxiv : the preprint server for biology · 2025Article
- Temperature-modulated separation of therapeutic cells, viral vectors, and exosomes using functional polymers.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2025Review
- Harvesting methods of umbilical cord-derived mesenchymal stem cells from culture modulate cell properties and functions.Regenerative therapy · 2024Article
- Bioanalytical technologies using temperature-responsive polymers.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2024Review
- Thermoresponsive bio-affinity interfaces for temperature-modulated selective capture and release of targeted exosomes.Materials today. Bio · 2023Article
- Chromatography columns packed with thermoresponsive-cationic-polymer-modified beads for therapeutic drug monitoring.Scientific reports · 2022Article
- Temperature-responsive mixed-mode column for the modulation of multiple interactions.Scientific reports · 2022Article
- A Molecular Shape Recognitive HPLC Stationary Phase Based on a Highly Ordered Amphiphilic Glutamide Molecular Gel.Nanomaterials (Basel, Switzerland) · 2021Article
- Effect of pore diameter on the elution behavior of analytes from thermoresponsive polymer grafted beads packed columns.Scientific reports · 2021Article
- Effective Separation for New Therapeutic Modalities Utilizing Temperature-responsive Chromatography.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2021Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Oligonucleotide therapeutics have contributed remarkably to healthcare, being well suited for the treatment of intractable diseases that are difficult to approach using conventional drug modalities. However, as common techniques of oligonucleotide analysis rely on reversed-phase or ion-exchange liquid chromatography and thus employ toxic organic solvents and/or ion-pairing reagents, better alternatives are highly sought after. Poly(N-isopropylacrylamide) (PNIPAAm) is widely used in temperature-responsive chromatography (TRC), which relies on column temperature variation to control the physical properties of the stationary phase and, unlike conventional reversed-phase liquid chromatography, avoids the use of toxic organic solvents and complicated gradient methods. Herein, PNIPAAm copolymer hydrogel-modified silica beads were used for the simultaneous analysis of multiple synthetic oligonucleotides by TRC to recognize differences in the length of single nucleotides, single bases, and the number of phosphorothioated sites. Temperature-responsive elution was observed in all cases. Each separation of all combinations of multiple oligonucleotides was better at higher temperatures above the lower critical solution temperature and was performed without the use of organic solvents and gradient methods. In the case of multiply phosphorothioated oligonucleotides, good separation was achieved using an aqueous solvent and isocratic elution in the absence of ion-pairing reagents. Thus, the developed procedure was concluded to be well suited for oligonucleotide analysis. Graphical abstract.
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