Evidence map›Paper›PMID 32528536›Full record

ArticleThe journal of venomous animals and toxins including tropical diseases2020

Extracellular vesicles in infectious diseases caused by protozoan parasites in buffaloes.

Leticia Gomes de Pontes, Wanessa Fernanda Altei, Asier Galan, Petra Bilić, Nicolas Guillemin, Josipa Kuleš, Anita Horvatić, Lígia Nunes de Morais Ribeiro, Eneida de Paula, Virgínia Bodelão Richini Pereira and 5 more

Open access · goldAbstract read
In one paragraph

Article in The journal of venomous animals and toxins including tropical diseases, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.2field-weighted citation impact, top 50% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 8 citations in OpenAlex.

  1. Pathogens (Basel, Switzerland) · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 6 institutions in 3 countries.

Leticia Gomes de PontesGraduate Program in Tropical Diseases, Botucatu Medical School (FMB), São Paulo State University (UNESP), Botucatu, SP, Brazil.ORCID 0000-0002-6648-3880
Wanessa Fernanda AlteiLaboratory of Biochemistry and Molecular Biology, Department of Physiological Sciences, Federal University of São Carlos (UFSCar), São Carlos, SP, Brazil.
Asier GalanERA Chair Team (VetMedZg), Clinic for Internal Diseases, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia.
Petra BilićERA Chair Team (VetMedZg), Clinic for Internal Diseases, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia.
Nicolas GuilleminERA Chair Team (VetMedZg), Clinic for Internal Diseases, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia.
Josipa KulešERA Chair Team (VetMedZg), Clinic for Internal Diseases, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia.
Anita HorvatićERA Chair Team (VetMedZg), Clinic for Internal Diseases, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia.
Lígia Nunes de Morais RibeiroDepartment of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, SP, Brazil.
Eneida de PaulaDepartment of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, SP, Brazil.
Virgínia Bodelão Richini PereiraAdolfo Lutz Institute, Center of Regional Laboratories II, Bauru, SP, Brazil.
Simone Baldini LucheisPaulista Agency of Agribusiness Technology (APTA), Bauru, SP, Brazil.
Vladimir MrljakERA Chair Team (VetMedZg), Clinic for Internal Diseases, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia.
Peter David EckersallERA Chair Team (VetMedZg), Clinic for Internal Diseases, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia.
Rui Seabra FerreiraGraduate Program in Tropical Diseases, Botucatu Medical School (FMB), São Paulo State University (UNESP), Botucatu, SP, Brazil.
Lucilene Delazari Dos SantosGraduate Program in Tropical Diseases, Botucatu Medical School (FMB), São Paulo State University (UNESP), Botucatu, SP, Brazil.ORCID 0000-0001-5832-1825
University of Zagreb · HRUniversidade Estadual Paulista (Unesp) · BRUniversidade Estadual de Campinas (UNICAMP) · BRAgência Paulista de Tecnologia dos Agronegócios · BRInstituto Adolfo Lutz · BRUniversidade Federal de São Carlos · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundExtracellular vesicles (EVs) are small membrane-bound vesicles of growing interest in vetetinary parasitology. The aim of the present report was to provide the first isolation, quantification and protein characterization of EVs from buffalo (

methodsInfected animals were identified through optical microscopy and PCR. EVs were isolated from buffalo sera by size-exclusion chromatography and characterized using western blotting analysis, nanoparticle tracking analysis and transmission electron microscopy. Subsequently, the proteins from isolated vesicles were characterized by mass spectrometry.

resultsEVs from buffalo sera have shown sizes in the 124-140 nm range and 306 proteins were characterized. The protein-protein interaction analysis has evidenced biological processes and molecular function associated with signal transduction, binding, regulation of metabolic processes, transport, catalytic activity and response to acute stress. Five proteins have been shown to be differentially expressed between the control group and that infected with

conclusionsEVs from buffaloes infected with

Indexed as

Extracellular vesiclesNanoparticle tracking analysisProteomic analysisProtozoan parasitesTheileria spp.

Identifiers

PMID32528536
PMCPMC7262785
OpenAlexW3034018162

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.