ArticleFrontiers in genetics2020
Deep-Sequencing Identification of MicroRNA Biomarkers in Serum Exosomes for Early Pig Pregnancy.
Article in Frontiers in genetics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 31 citations in OpenAlex.
- Article
- Screening and analysis of plasma small extracellular vesicle MiRNAs at various estrous cycle phases of goat.Journal of ovarian research · 2025Article
- Expanding Horizons: Next-Generation and Interdisciplinary Advances in the Applications of Extracellular Vesicles.Journal of extracellular biology · 2025Review
- MicroRNA (miRNA) in Plasma Small Extracellular Vesicles (sEV) as Potential Early Indicators of Dairy Cow Subfertility.Journal of extracellular biology · 2025Article
- Metabolomics reveals early pregnancy biomarkers in sows: a non-invasive diagnostic approach.Frontiers in veterinary science · 2024Article
- An intelligent method for pregnancy diagnosis in breeding sows according to ultrasonography algorithms.Journal of animal science and technology · 2023Article
- MicroRNAs in Ruminants and Their Potential Role in Nutrition and Physiology.Veterinary sciences · 2023Review
- Short communication: profiling the expression of Let-7d-5p microRNA in circulating blood of pregnant and nonpregnant cows.Journal of animal science · 2023Article
- Ssc-miR-92b-3p Regulates Porcine Trophoblast Cell Proliferation and Migration via theInternational journal of molecular sciences · 2022Article
- Extracellular vesicles derived from endometrial epithelial cells deliver exogenous miR-92b-3p to affect the function of embryonic trophoblast cells via targeting TSC1 and DKK3.Reproductive biology and endocrinology : RB&E · 2022Article
- Extracellular Vesicles in Veterinary Medicine.Animals : an open access journal from MDPI · 2022Review
- Reactive oxygen and nitrogen species regulate porcine embryo development during pre-implantation period: A mini-review.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2021Review
- Insights Into Extracellular Vesicle/Exosome and miRNA Mediated Bi-Directional Communication During Porcine Pregnancy.Frontiers in veterinary science · 2021Review
- MicroRNAs as Biomarkers for Animal Health and Welfare in Livestock.Frontiers in veterinary science · 2020Review
Corrections and comments
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Authors and funding
13 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Early pregnancy diagnosis in sows can significantly improve the efficiency of pig industry. Exosomes are membrane-covered nanovesicles that can transport microRNAs (miRNAs) and other molecular signals between cells. In other species, serum exosome-derived miRNAs can serve as good biomarkers of diseases and different physiological states, including pregnancy status. We hypothesized that circulating exosome-derived miRNAs might be used to differentiate the pregnancy status as early as several days after insemination in pigs. To test this hypothesis, we randomly assigned pigs for artificial insemination with fertile or dead semen (control group). Serum samples were obtained from pregnant pigs on days 9, 12, and 15 after insemination and from non-pregnant pigs on days 0, 9, 12, and 15 after insemination. Exosomes were isolated for RNA extraction. The exosomal RNA samples from pigs on day 9 of the estrus cycle and pregnancy were used for small-RNA sequencing. A total 321 miRNAs were identified in all samples. Twenty eight differentially abundant miRNAs were identified between the pregnant and control groups. miRNAs with | log2 (fold change)| > 2 from sequencing results were selected for validation by quantitative reverse-transcription-polymerase chain reaction (RT-qPCR) in larger samples. Finally two upregulated miRNAs (miR-92b-3p and miR-17-5p) in the pregnant groups (on days 9, 12, and 15 of pregnancy) were confirmed by RT-qPCR. In summary, we have successfully identified circulating exosomal miRNA profiles in the serum of pigs in early pregnancy. miR-92b-3p and miR-17-5p could be used as potential circulating biomarkers for early pregnancy diagnosis.
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