ArticleFrontiers in microbiology2020
Genome Size, rDNA Copy, and qPCR Assays for Symbiodiniaceae.
Article in Frontiers in microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Coral-associated microbiome dynamics under thermal and pollution stress.Conservation biology : the journal of the Society for Conservation Biology · 2026Article
- Stress-Resistant Symbiodiniaceae and Diverse Bacterial Communities Promote Coral Persistence in Variable, Multi-Stressor Environments.Ecology and evolution · 2026Article
- Influence of Habitat Alteration on the Molecular Profile of Membrane Lipids of the CoralBiology · 2026Article
- Runaway coral-algal dysbiosis may be responsible for rapid coral tissue loss.Scientific reports · 2026Article
- Microbial dynamics in coral reef waters: Diel cycles in contrasting seasons.Science advances · 2026Article
- Key coral symbiont lineages (family Symbiodiniaceae) are differentially associated with stony coral tissue loss disease.ISME communications · 2026Article
- Exposure to marine contaminant mixtures with different toxicity drivers reduces microzooplankton diversity.FEMS microbiology ecology · 2025Article
- The Ribosomal Operon Database: A Full-Length rDNA Operon Database Derived From Genome Assemblies.Molecular ecology resources · 2025Article
- Review
- Highly Diverse Symbiodiniaceae Types Hosted by Corals in a Global Hotspot of Marine Biodiversity.Microbial ecology · 2024Article
- Massive genome reduction predates the divergence of Symbiodiniaceae dinoflagellates.The ISME journal · 2024Article
- Filamentous virus-like particles are present in coral dinoflagellates across genera and ocean basins.The ISME journal · 2023Article
- Multi-omics analysis reveals the molecular response to heat stress in a "red tide" dinoflagellate.Genome biology · 2023Article
- Mock community experiments can inform on the reliability of eDNA metabarcoding data: a case study on marine phytoplankton.Scientific reports · 2023Article
- Cellular traits regulate fluorescence-based light-response phenotypes of coral photosymbionts livingFrontiers in physiology · 2023Article
- Building consensus around the assessment and interpretation of Symbiodiniaceae diversity.PeerJ · 2023Article
- Gene clusters for biosynthesis of mycosporine-like amino acids in dinoflagellate nuclear genomes: Possible recent horizontal gene transfer between species of Symbiodiniaceae (Dinophyceae).Journal of phycology · 2022Review
- Octocorals in the Gulf of Aqaba exhibit high photosymbiont fidelity.Frontiers in microbiology · 2022Article
- Comparison of 15 dinoflagellate genomes reveals extensive sequence and structural divergence in family Symbiodiniaceae and genus Symbiodinium.BMC biology · 2021Article
- Homogenization of Endosymbiont Communities Hosted by Equatorial Corals during the 2016 Mass Bleaching Event.Microorganisms · 2020Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Symbiodiniaceae community structure in corals is crucial for understanding the plasticity of different holobionts under environmental stress. While this relies on molecular analyses, accuracy of molecular quantification, as influenced by DNA extraction efficiency and rDNA copy number variations in particular, has rarely been systematically investigated. Here, we report the development of a set of genus-specific qPCR assays. First, a protocol for efficient DNA isolation and accurate measurements of genome size and rDNA copy number was established. Second, seven newly designed genus-specific ITS2 primer sets were validated using computational and empirical analyses and qPCR assays were developed. We find that while the genome size ranges between 1.75 ± 0.21 and 4.5 ± 0.96 Gbp, rDNA copy number shows over 10-fold variation among Symbiodiniaceae species. Our protocol produced standard curves with high efficiencies (89.8-99.3%;
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.