ArticleClinical proteomics2020
Human follicular fluid proteome reveals association between overweight status and oocyte maturation abnormality.
Article in Clinical proteomics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 16 citations in OpenAlex.
- Treatment of human oocytes with extracellular vesicles from follicular fluid during rescueHuman reproduction open · 2026Article
- An exploratory single arm study to evaluate the role of an Ayurvedic treatment protocol as a prerequisite forFrontiers in medicine · 2025Article
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- The impact of obesity on reproductive health and metabolism in reproductive-age females.Fertility and sterility · 2024Review
- Cellular atlas of the human ovary using morphologically guided spatial transcriptomics and single-cell sequencing.Science advances · 2024Article
- In vitro growth (IVG) of human ovarian follicles in frozen thawed ovarian cortex tissue culture supplemented with follicular fluid under hypoxic conditions.Archives of gynecology and obstetrics · 2022Article
- Early programming of reproductive health and fertility: novel neuroendocrine mechanisms and implications in reproductive medicine.Human reproduction update · 2022Review
- Distinct immune and inflammatory response patterns contribute to the identification of poor prognosis and advanced clinical characters in bladder cancer patients.Frontiers in immunology · 2022Article
- Proteome of fluid from human ovarian small antral follicles reveals insights in folliculogenesis and oocyte maturation.Human reproduction (Oxford, England) · 2021Article
- Proteomic Analysis of the Alterations in Follicular Fluid Proteins During Oocyte Maturation in Humans.Frontiers in endocrinology · 2021Article
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Authors and funding
12 authors at 4 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundHuman follicular fluid (HFF), which is composed by essential proteins required for the follicle development, provides an important microenvironment for oocyte maturation. Recently, overweight status has been considered as a detrimental impact factor on oocyte maturation, but whether HFF proteome could provide protein markers for assessing overweight-based oocyte maturation deficiency is still unknown.
methodsTo reveal the HFF-based molecular characteristics associated with abnormal oocyte maturation, an iTRAQ-based comparative proteomic analysis was performed to investigate different HFF protein expression profiles from normal weight women and overweight status women.
resultsTwo hundred HFF proteins were quantified in our data, of which 43% have not been overlapped by two previous publications. Compared with the HFF proteins of normal weight women, 22 up-regulated HFF proteins and 21 down-regulated HFF proteins were found in the overweight status women. PANTHER database showed these altered HFF proteins participated in development, metabolism, immunity, and coagulation, and STRING database demonstrated their complicated interaction networks. The confidence of proteomic outcome was verified by Western blot analysis of WAP four-disulfide core domain protein 2 (WFDC2), lactotransferrin (LTF), prostate-specific antigen (KLK3), fibronectin (FN1), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH). Further, ELISA assay indicated WFDC2 might be a potentially useful candidate HFF marker for the diagnosis of oocyte maturation arrest caused by overweight status.
conclusionsOur work provided a new complementary high-confidence HFF dataset involved in oocyte maturation, and these altered HFF proteins might have clinical relevance and diagnostic and prognostic value for abnormal oocyte maturation in overweight status women.
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