ArticleScientific reports2020
Detachment of cell sheets from clinically ubiquitous cell culture vessels by ultrasonic vibration.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Article
- 3D printed nano-hydroxyapatite scaffold combined with BMSCs cell sheet containing concentrated growth factor (CGF) for enhancing bone regeneration.Stem cell research & therapy · 2026Article
- Fabrication of a low-adhesion metallic cell culture surface by nanosecond laser processing.Bioprocess and biosystems engineering · 2026Article
- Scaffold-free cell sheet therapies: clinical advances, global approval landscapes, and strategic directions to address regenerative medicine barriers.Stem cell research & therapy · 2025Review
- Engineering Extracellular Microenvironments: The Impact of Fibrous Materials on Cell Behavior.Advanced healthcare materials · 2025Review
- Thermoresponsive BrushGel Microcarriers for Efficient Cell Expansion and Enzyme-Reduced Harvesting.Advanced healthcare materials · 2025Article
- Influence of Exposure Time and Driving Frequency on Cytotoxicity in In Vitro Ultrasound With Constant Mechanical Indices.Engineering in life sciences · 2025Article
- Scalable fabrication, compartmentalization and applications of living microtissues.Bioactive materials · 2023Article
- Well-free agglomeration and on-demand three-dimensional cell cluster formation using guided surface acoustic waves through a couplant layer.Biomedical microdevices · 2022Article
- Cell Sheet Technology as an Engineering-Based Approach to Bone Regeneration.International journal of nanomedicine · 2022Review
- Molecular and Metabolic Mechanism of Low-Intensity Pulsed Ultrasound Improving Muscle Atrophy in Hindlimb Unloading Rats.International journal of molecular sciences · 2021Article
- Development of accurate temperature regulation culture system with metallic culture vessel demonstrates different thermal cytotoxicity in cancer and normal cells.Scientific reports · 2021Article
- Travelling ultrasound promotes vasculogenesis of three-dimensional-monocultured human umbilical vein endothelial cells.Biotechnology and bioengineering · 2021Article
- Mechanical properties of cell sheets and spheroids: the link between single cells and complex tissues.Biophysical reviews · 2021Review
- Fundamental Technologies and Recent Advances of Cell-Sheet-Based Tissue Engineering.International journal of molecular sciences · 2021Review
- Propagating acoustic waves on a culture substrate regulate the directional collective cell migration.Microsystems & nanoengineering · 2021Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Proteinases that digest the extracellular matrix are usually used to harvest cells from culture vessels in a general culture process, which lowers the initial adhesion rate in regenerative medicine. Cell sheet engineering is one of the most important technologies in this field, especially for transplantation, because fabricated cell sheets have rich extracellular matrixes providing strong initial adhesion. Current cell sheet fabrication relies on temperature-responsive polymer-coated dishes. Cells are cultured on such specialized dishes and subjected to low temperature. Thus, we developed a simple but versatile cell sheet fabrication method using ubiquitous culture dishes/flasks without any coating or temperature modulation. Confluent mouse myoblasts (C2C12 cell line) were exposed to ultrasonic vibration from underneath and detached as cell sheets from entire culture surfaces. Because of the absence of low temperature, cell metabolism was statically increased compared with the conventional method. Furthermore, viability, morphology, protein expression, and mRNA expression were normal. These analyses indicated no side effects of ultrasonic vibration exposure. Therefore, this novel method may become the standard for cell sheet fabrication. Our method can be easily conducted following a general culture procedure with a typical dish/flask, making cell sheets more accessible to medical experts.
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