ArticleMicrobial biotechnology2020
Revisiting the host adhesion determinants of Streptococcus thermophilus siphophages.
Article in Microbial biotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 28 citations in OpenAlex.
- Dynamic adhesion device of phage OE33PA drives Gram-positive host recognition.bioRxiv : the preprint server for biology · 2026Article
- Diversity and prevalence of dairy streptococcal phages in two European dairy fermentation facilities over a one-year period.Microbial genomics · 2026Article
- Functional Evaluation of a Conserved HNH Endonuclease Gene in Lactococcal Skunavirus Genomes.Microbial biotechnology · 2026Article
- The cell surface-associated rhamnose-glucose polysaccharide represents the receptor ofApplied and environmental microbiology · 2025Article
- Isolation of phages infecting the zoonotic pathogenbioRxiv : the preprint server for biology · 2025Article
- Functional and practical insights into three lactococcal antiphage systems.Applied and environmental microbiology · 2024Article
- Deciphering the adsorption machinery of Deep-Blue and Vp4, two myophages targeting members of theJournal of virology · 2024Article
- Partial Atomic Model of the Tailed Lactococcal Phage TP901-1 as Predicted by AlphaFold2: Revelations and Limitations.Viruses · 2023Article
- Bacteriophage-host interactions in Streptococcus thermophilus and their impact on co-evolutionary processes.FEMS microbiology reviews · 2023Review
- Article
- Exploring Host-Binding Machineries of Mycobacteriophages with AlphaFold2.Journal of virology · 2023Article
- Genetic determinants of host tropism in Klebsiella phages.Cell reports · 2023Article
- Phage Adsorption to Gram-Positive Bacteria.Viruses · 2023Review
- Exploring Structural Diversity among Adhesion Devices Encoded by Lactococcal P335 Phages with AlphaFold2.Microorganisms · 2022Article
- Viral Proteins Involved in the Adsorption Process of Deep-Purple, a Siphovirus Infecting Members of the Bacillus cereus Group.Applied and environmental microbiology · 2022Article
- Article
- Brussowvirus SW13 Requires a Cell Surface-Associated Polysaccharide To Recognize Its Streptococcus thermophilus Host.Applied and environmental microbiology · 2022Article
- Bacteriophage-host interactions as a platform to establish the role of phages in modulating the microbial composition of fermented foods.Microbiome research reports · 2022Review
- A structural discovery journey of streptococcal phages adhesion devices by AlphaFold2.Frontiers in molecular biosciences · 2022Article
- Structure and Topology Prediction of Phage Adhesion Devices Using AlphaFold2: The Case of TwoMicroorganisms · 2021Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Available 3D structures of bacteriophage modules combined with predictive bioinformatic algorithms enabled the identification of adhesion modules in 57 siphophages infecting Streptococcus thermophilus (St). We identified several carbohydrate-binding modules (CBMs) in so-called evolved distal tail (Dit) and tail-associated lysozyme (Tal) proteins of St phage baseplates. We examined the open reading frame (ORF) downstream of the Tal-encoding ORF and uncovered the presence of a putative p2-like receptor-binding protein (RBP). A 21 Å resolution electron microscopy structure of the baseplate of cos-phage STP1 revealed the presence of six elongated electron densities, surrounding the core of the baseplate, that harbour the p2-like RBPs at their tip. To verify the functionality of these modules, we expressed GFP- or mCherry-coupled Tal and putative RBP CBMs and observed by fluorescence microscopy that both modules bind to their corresponding St host, the putative RBP CBM with higher affinity than the Tal-associated one. The large number of CBM functional domains in St phages suggests that they play a contributory role in the infection process, a feature that we previously described in lactococcal phages and beyond, possibly representing a universal feature of the siphophage host-recognition apparatus.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.