Kinetic analysis of the intracellular processing of siRNAs by confocal microscopy.

Daniel Vocelle, Olivia M Chesniak, Milton R Smith, Christina Chan, S Patrick Walton

Abstract read

In one paragraph

Article in Microscopy (Oxford, England), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Daniel VocelleDepartment of Chemical Engineering and Materials Science, Michigan State University, 428 S Shaw Lane, East Lansing, MI 48824, USA.

Olivia M ChesniakDepartment of Chemistry, Michigan State University, 578 S Shaw Lane, East Lansing, MI 48824, USA.

Milton R SmithDepartment of Chemistry, Michigan State University, 578 S Shaw Lane, East Lansing, MI 48824, USA.

Christina ChanDepartment of Chemical Engineering and Materials Science, Michigan State University, 428 S Shaw Lane, East Lansing, MI 48824, USA.

S Patrick WaltonDepartment of Chemical Engineering and Materials Science, Michigan State University, 428 S Shaw Lane, East Lansing, MI 48824, USA.

Funding

Maximizing siRNA Function through Mechanism-based Sequence and Vehicle DesignR01GM089866 · NIGMS · MICHIGAN STATE UNIVERSITY · PI WALTON, STEPHEN PATRICK · 2010 to 2013

$922k

NIGMS NIH HHS R01 GM089866

6 · The paper itself

Abstract

Here, we describe a method for tracking intracellular processing of small interfering RNA (siRNA) containing complexes using automated microscopy controls and image acquisition to minimize user effort and time. This technique uses fluorescence colocalization to monitor dual-labeled fluorescent siRNAs delivered by silica nanoparticles in different intracellular locations, including the early/late endosomes, fast/slow recycling endosomes, lysosomes and the endoplasmic reticulum. Combining the temporal association of siRNAs with each intracellular location, we reconstructed the intracellular pathways used in siRNA processing, and demonstrate how these pathways vary based on the chemical composition of the delivery vehicle.

Indexed as

Endoplasmic ReticulumEndosomesHeLa CellsHumansKineticsLysosomesMicroscopy, ConfocalRNA, Small InterferingRNA, Small Interferingconfocal microscopydelivery vehiclesHeLaintracellular traffickingkineticssiRNA

Identifiers

PMID32525202

DOI10.1093/jmicro/dfaa031

PMCPMC7711900

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.