ArticleScience advances2020
A versatile depigmentation, clearing, and labeling method for exploring nervous system diversity.
Article in Science advances, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
59 citing papers in PubMed.
- Multiplexed gene expression analysis in dissociated and intact planarian flatworm tissues using in situ hybridization chain reaction.Biology open · 2026Article
- Whole-body single-cell atlas of an adult vertebrate in homeostasis and regeneration.bioRxiv : the preprint server for biology · 2026Article
- The origin and evolution of dorsal determination mechanisms in vertebrate paired appendages.Current biology : CB · 2026Article
- A uniform tissue-clearing framework and mesoSPIM-ultra enable cm-scale single-neuron tracing.bioRxiv : the preprint server for biology · 2026Article
- activeDISCO enables large-scale exploration of human brain anatomy by light sheet microscopy.Communications biology · 2026Article
- Root tip excision-induced exodermis lignification impacts lateral root emergence in Brachypodium distachyon.The New phytologist · 2026Article
- Early motor deficits, sleep dysfunction and reduction in dopaminergic neurons in a PARK7Scientific reports · 2026Article
- A spatiotemporal atlas of cerebrovascular development in zebrafish.Nature communications · 2026Article
- Zebrafish genetic model of neuromuscular degeneration associated with Atrogin-1 expression.PLoS genetics · 2026Article
- Innervation Drives Postembryonic Expansion of the Zebrafish Anterior Lateral Line System.The Journal of comparative neurology · 2026Article
- A novelFrontiers in cell and developmental biology · 2026Article
- The nascent RNA labelling compound 5-ethynyl uridine (EU) integrates into DNA in some animals.BMC molecular and cell biology · 2025Article
- Enhancer-directed gene delivery for digit regeneration based on conserved epidermal factors.bioRxiv : the preprint server for biology · 2025Article
- Rapid sonication-assisted whole tissue clearing and immunostaining.Scientific reports · 2025Article
- Rationally Designed TadA-Derived Cytosine Editors Enable Context-Independent Zebrafish Genome Editing.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Heparanase 2 Modulates Vascular Permeability via Heparan Sulfate-Dependent Growth Factor Signaling.Arteriosclerosis, thrombosis, and vascular biology · 2025Article
- OpenEMMU: A versatile, open-source EdU multiplexing methodology for studying DNA replication and cell cycle dynamics.iScience · 2025Article
- Development of the zebrafish anterior lateral line system is influenced by underlying cranial neural crest.Developmental biology · 2025Article
- Dopaminergic Neurons in the Zebrafish Subpallium Belong to the Extended Medial Amygdala.The Journal of comparative neurology · 2025Article
- Comprehensive 3D Imaging of Whole Zebrafish Using a Water-Based Clearing Reagent for Hard Tissues.Zebrafish · 2025Article
Corrections and comments
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Authors and funding
16 authors.
Funding
Abstract
Tissue clearing combined with deep imaging has emerged as a powerful alternative to classical histological techniques. Whereas current techniques have been optimized for imaging selected nonpigmented organs such as the mammalian brain, natural pigmentation remains challenging for most other biological specimens of larger volume. We have developed a fast DEpigmEntation-Plus-Clearing method (DEEP-Clear) that is easily incorporated in existing workflows and combines whole system labeling with a spectrum of detection techniques, ranging from immunohistochemistry to RNA in situ hybridization, labeling of proliferative cells (EdU labeling) and visualization of transgenic markers. With light-sheet imaging of whole animals and detailed confocal studies on pigmented organs, we provide unprecedented insight into eyes, whole nervous systems, and subcellular structures in animal models ranging from worms and squids to axolotls and zebrafish. DEEP-Clear thus paves the way for the exploration of species-rich clades and developmental stages that are largely inaccessible by regular imaging approaches.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.