Evidence map›Paper›PMID 32521655›Full record

ArticleMolecules (Basel, Switzerland)2020

Signature Ions in MS/MS Spectra for Dansyl-Aminohexyl-QQIV Adducts on Lysine.

Lawrence M Schopfer, Oksana Lockridge

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecules (Basel, Switzerland), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Lawrence M SchopferEppley Institute, University of Nebraska Medical Center, Omaha, NE 68198, USA.
Oksana LockridgeEppley Institute, University of Nebraska Medical Center, Omaha, NE 68198, USA.ORCID 0000-0002-8345-3640

Funding

UNMC/EPPLEY CANCER CENTER SUPPORT GRANTP30CA036727 · NCI · UNIVERSITY OF NEBRASKA MEDICAL CENTER · PI James Eudy · 1985 to 2026
$55.0M
Crosslinking Action of Chlorpyrifos Oxon as Mechanism of Chronic IllnessR21ES030132 · NIEHS · UNIVERSITY OF NEBRASKA MEDICAL CENTER · PI LOCKRIDGE, OKSANA · 2020 to 2021
$419k
NCI NIH HHS P30 CA036727NIEHS NIH HHS R21 ES030132NIH HHS 1R21ES030132-01A1NIH HHS P30CA036727
6 · The paper itself

Abstract

Bacterial transglutaminase was used to label human plasma proteins with fluorescent tags. Protein lysines were modified with dansyl-epsilon-aminohexyl-Gln-Gln-Ile-Val-OH (dansylQQIV), while protein glutamines were modified with dansyl cadaverine. Labeled proteins included human butyrylcholinesterase, apolipoprotein A-1, haptoglobin, haptoglobin-related protein, immunoglobulin heavy chain, and hemopexin. Tryptic peptides were analyzed by LC-MS/MS on an Orbitrap Fusion Lumos mass spectrometer. Modified residues were identified in Protein Prospector and Proteome Discoverer searches of mass spectrometry data. The MS/MS fragmentation spectra from dansylQQIV-modified peptides gave intense peaks at 475.2015, 364.1691, 347.1426, 234.0585, and 170.0965

Indexed as

ButyrylcholinesteraseCadaverineDansyl CompoundsGlutamineHumansLysineTandem Mass SpectrometryBCHE protein, humanButyrylcholinesteraseCadaverineDansyl CompoundsGlutamineLysinebutyrylcholinesterasedansylQQIVmass spectrometryplasma proteinsProtein Prospectortransglutaminase

Identifiers

PMID32521655
PMCPMC7321351

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.