Evidence map›Paper›PMID 32521604›Full record

ArticleBiomolecules2020

High-Throughput Identification of Adapters in Single-Read Sequencing Data.

Asan M S H Mohideen, Steinar D Johansen, Igor Babiak

Open access · goldAbstract read
In one paragraph

Article in Biomolecules, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
0.3field-weighted citation impact, top 47% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 14 citations in OpenAlex.

  1. Article
  2. Genome-wide selective sweep analysis in high-altitude Changthangi goats reveals candidate genes for pashmina fiber production.Mammalian genome : official journal of the International Mammalian Genome Society · 2025
    Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Discovering a novel glycosyltransferase geneFrontiers in microbiology · 2024
    Article
  8. Article
  9. Article
  10. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Asan M S H MohideenGenomics Group, Faculty of Biosciences and Aquaculture, Nord University, P.O. Box 1490, 8049 Bodø, Norway.
Steinar D JohansenGenomics Group, Faculty of Biosciences and Aquaculture, Nord University, P.O. Box 1490, 8049 Bodø, Norway.
Igor BabiakGenomics Group, Faculty of Biosciences and Aquaculture, Nord University, P.O. Box 1490, 8049 Bodø, Norway.ORCID 0000-0002-1462-2690
Nord University · NO

Funding

Norges Forskningsråd 275786
6 · The paper itself

Abstract

Sequencing datasets available in public repositories are already high in number, and their growth is exponential. Raw sequencing data files constitute a substantial portion of these data, and they need to be pre-processed for any downstream analyses. The removal of adapter sequences is the first essential step. Tools available for the automated detection of adapters in single-read sequencing protocol datasets have certain limitations. To explore these datasets, one needs to retrieve the information on adapter sequences from the methods sections of appropriate research articles. This can be time-consuming in metadata analyses. Moreover, not all research articles provide the information on adapter sequences. We have developed adapt_find, a tool that automates the process of adapter sequences identification in raw single-read sequencing datasets. We have verified adapt_find through testing a number of publicly available datasets. adapt_find secures a robust, reliable and high-throughput process across different sequencing technologies and various adapter designs. It does not need prior knowledge of the adapter sequences. We also produced associated tools: random_mer, for the detection of random N bases either on one or both termini of the reads, and fastqc_parser, for consolidating the results from FASTQC outputs. Together, this is a valuable tool set for metadata analyses on multiple sequencing datasets.

Indexed as

High-Throughput Nucleotide SequencingSequence Analysis, DNAOligonucleotidesOligonucleotides454 pyrosequencingadapter oligonucleotidesadapter trimmingIlluminaIon-Torrentrandomized adapterssingle-read sequencingsmall RNA sequencingSOLiD

Identifiers

PMID32521604
PMCPMC7356586
OpenAlexW3032927183

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.