Evidence map›Paper›PMID 32521262›Full record

ArticleCell reports2020

Engineered Illumination Devices for Optogenetic Control of Cellular Signaling Dynamics.

Nicole A Repina, Thomas McClave, Hunter J Johnson, Xiaoping Bao, Ravi S Kane, David V Schaffer

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.

0numbers the graph read from it
0cells of the map it votes in
48citing papers in PubMed
5.3field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

48 citing papers in PubMed, 72 citations in OpenAlex.

  1. A practical toolbox for modelling fibrosis in vitro.Nature biomedical engineering · 2026
    Review
  2. Article
  3. Article
  4. Review
  5. Article
  6. Article
  7. Article
  8. Article
  9. Optogenetic control of Nodal signaling patterns.Development (Cambridge, England) · 2025
    Article
  10. Article
  11. Targeted Activation of Programmed Cell Death Pathways by Optogenetics.Methods in molecular biology (Clifton, N.J.) · 2025
    Article
  12. Modular Light-Emitting Diode Shelving Systems for Scalable Optogenetics.Methods in molecular biology (Clifton, N.J.) · 2025
    Article
  13. Article
  14. Article
  15. Optogenetic control of Nodal signaling patterns.bioRxiv : the preprint server for biology · 2024
    Article
  16. Article
  17. Article
  18. Review
  19. Optogenetic clustering and membrane translocation of the BcLOV4 photoreceptor.Proceedings of the National Academy of Sciences of the United States of America · 2023
    Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Nicole A RepinaDepartment of Bioengineering, University of California, Berkeley, Berkeley, CA 94720, USA; Graduate Program in Bioengineering, University of California, San Francisco, San Francisco, CA, USA; Graduate Program in Bioengineering, University of California, Berkeley, Berkeley, CA 94720, USA.
Thomas McClaveDepartment of Physics, University of California, Berkeley, Berkeley, CA 94720, USA.
Hunter J JohnsonDepartment of Bioengineering, University of California, Berkeley, Berkeley, CA 94720, USA; Graduate Program in Bioengineering, University of California, San Francisco, San Francisco, CA, USA; Graduate Program in Bioengineering, University of California, Berkeley, Berkeley, CA 94720, USA.
Xiaoping BaoDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, Berkeley, CA 94720, USA.
Ravi S KaneSchool of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA. Electronic address: ravi.kane@chbe.gatech.edu.
David V SchafferDepartment of Bioengineering, University of California, Berkeley, Berkeley, CA 94720, USA; Department of Chemical and Biomolecular Engineering, University of California, Berkeley, Berkeley, CA 94720, USA; Helen Wills Neuroscience Institute, University of California, Berkeley, Berkeley, CA 94720, USA; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA. Electronic address: schaffer@berkeley.edu.
University of California, Berkeley · USGeorgia Institute of Technology · US

Funding

Engineer Biomimetic Microfluidic Models to Investigate and Reprogram Tumor Associated Neutrophils for Cancer TherapyR37CA265926 · NCI · PURDUE UNIVERSITY · PI Xiaoping Bao · 2022 to 2026
$1.7M
Optogenetic Characterization and Control of Stem Cell SignalingR01NS087253 · NINDS · UNIVERSITY OF CALIFORNIA BERKELEY · PI KANE, RAVI S., SCHAFFER, DAVID V · 2014 to 2018
$1.7M
CAR neutrophils produced in vivo to remodel tumor microenvironment and treat glioblastomaR01CA293514 · NCI · PURDUE UNIVERSITY · PI Xiaoping Bao, Qing Deng · 2025 to 2026
$1.3M
NCI NIH HHS R01 CA293514NCI NIH HHS R37 CA265926NINDS NIH HHS R01 NS087253
6 · The paper itself

Abstract

Spatially and temporally varying patterns of morphogen signals during development drive cell fate specification at the proper location and time. However, current in vitro methods typically do not allow for precise, dynamic spatiotemporal control of morphogen signaling and are thus insufficient to readily study how morphogen dynamics affect cell behavior. Here, we show that optogenetic Wnt/β-catenin pathway activation can be controlled at user-defined intensities, temporal sequences, and spatial patterns using engineered illumination devices for optogenetic photostimulation and light activation at variable amplitudes (LAVA). By patterning human embryonic stem cell (hESC) cultures with varying light intensities, LAVA devices enabled dose-responsive control of optoWnt activation and Brachyury expression. Furthermore, time-varying and spatially localized patterns of light revealed tissue patterning that models the embryonic presentation of Wnt signals in vitro. LAVA devices thus provide a low-cost, user-friendly method for high-throughput and spatiotemporal optogenetic control of cell signaling for applications in developmental and cell biology.

Indexed as

Cell DifferentiationEmbryonic Stem CellsHumansOptogeneticsSignal Transductioncanonical Wntcellular signalingdifferentiationelectronics designhuman embryonic stem cellsmesendodermoptogeneticsspatiotemporal dynamicstissue patterning

Identifiers

PMID32521262
PMCPMC9357365
OpenAlexW3033184890

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.