ArticleThe New phytologist2020
The Medicago truncatula Vacuolar iron Transporter-Like proteins VTL4 and VTL8 deliver iron to symbiotic bacteria at different stages of the infection process.
Article in The New phytologist, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Long-Distance Trafficking of Mineral Elements From Soil-to-Seed Under Climate Change Impacts Crop Nutritional Quality.Plant, cell & environment · 2026Review
- Molecular mechanisms modulating beneficial plant root-microbe interactions: What's common?Plant communications · 2026Review
- Review
- A node-localized efflux transporter for loading iron to developing tissues in rice.Nature communications · 2025Article
- Identification, characterization, and function analysis of the VIT family in Phaeodactylum tricornutum.Scientific reports · 2025Article
- Nutrient-dependent regulation of symbiotic nitrogen fixation in legumes.Horticulture research · 2025Article
- The Rhizophagus irregularis permease RiFTR1 functions without a ferroxidase partner for reductive iron transport.Scientific reports · 2025Article
- Arabidopsis MEB3 functions as a vacuolar metal transporter to regulate iron accumulation in roots.Frontiers in plant science · 2025Article
- Understanding the Crucial Role of Phosphate and Iron Availability in Regulating Root Nodule Symbiosis.Plant & cell physiology · 2024Review
- Inorganic nitrogen inhibits symbiotic nitrogen fixation through blocking NRAMP2-mediated iron delivery in soybean nodules.Nature communications · 2024Article
- Metal nutrition and transport in the process of symbiotic nitrogen fixation.Plant communications · 2024Review
- Defense and senescence interplay in legume nodules.Plant communications · 2024Review
- Editorial: Plant-rhizobia symbiosis and nitrogen fixation in legumes.Frontiers in plant science · 2024Article
- Genetic variation and genetic complexity of nodule occupancy in soybean inoculated with USDA110 and USDA123 rhizobium strains.BMC genomics · 2023Article
- The vacuolar iron transporter mediates iron detoxification in Toxoplasma gondii.Nature communications · 2023Article
- Iron in the Symbiosis of Plants and Microorganisms.Plants (Basel, Switzerland) · 2023Review
- Insight into the control of nodule immunity and senescence during Medicago truncatula symbiosis.Plant physiology · 2023Article
- Expression and mutagenesis studies in theFrontiers in plant science · 2023Article
- Innovation and appropriation in mycorrhizal and rhizobial Symbioses.The Plant cell · 2022Article
- Time Series Transcriptome Analysis inFrontiers in plant science · 2022Article
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Authors and funding
9 authors.
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Abstract
The symbiotic relationship between legumes and rhizobium bacteria in root nodules has a high demand for iron, and questions remain regarding which transporters are involved. Here, we characterize two nodule-specific Vacuolar iron Transporter-Like (VTL) proteins in Medicago truncatula. Localization of fluorescent fusion proteins and mutant studies were carried out to correlate with existing RNA-seq data showing differential expression of VTL4 and VTL8 during early and late infection, respectively. The vtl4 insertion lines showed decreased nitrogen fixation capacity associated with more immature nodules and less elongated bacteroids. A mutant line lacking the tandemly-arranged VTL4-VTL8 genes, named 13U, was unable to develop functional nodules and failed to fix nitrogen, which was almost fully restored by expression of VTL8 alone. Using a newly developed lux reporter to monitor iron status of the bacteroids, a moderate decrease in luminescence signal was observed in vtl4 mutant nodules and a strong decrease in 13U nodules. Iron transport capability of VTL4 and VTL8 was shown by yeast complementation. These data indicate that VTL8, the closest homologue of SEN1 in Lotus japonicus, is the main route for delivering iron to symbiotic rhizobia. We propose that a failure in iron protein maturation leads to early senescence of the bacteroids.
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