ArticleNucleic acids research2020
CLICK-17, a DNA enzyme that harnesses ultra-low concentrations of either Cu+ or Cu2+ to catalyze the azide-alkyne 'click' reaction in water.
Article in Nucleic acids research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 30 citations in OpenAlex.
- 1,2,3-Triazole-linked DNA biosensors: molecular design to real-time applications.RSC advances · 2026Review
- COF@DNAzyme Empowering Endogenous Copper for One-Stitch Bioorthogonal Catalysis-Based Anticancer Therapy.Angewandte Chemie (International ed. in English) · 2026Article
- Retrosynthetic Design of Dinuclear Copper Enzymes for Azide-Alkyne Cycloaddition via Clickable Noncanonical Amino Acids.Journal of the American Chemical Society · 2025Article
- Copper-catalysed azide-alkyne cycloaddition on live M13 bacteriophage for expanding the molecular diversity of phage-displayed peptide libraries.RSC chemical biology · 2025Article
- Click DNA ligation with deoxyribozyme.Nucleic acids research · 2025Article
- Multidomain Molecular Sensor Devices, Systems, and Algorithms for Improved Physiological Monitoring.Micromachines · 2025Review
- Aptamer and DNAzyme Based Colorimetric Biosensors for Pathogen Detection.Angewandte Chemie (International ed. in English) · 2025Review
- Biomolecular sensors for advanced physiological monitoring.Nature reviews bioengineering · 2023Review
- Preparation, applications, and challenges of functional DNA nanomaterials.Nano research · 2023Review
- A DNAzyme-augmented bioorthogonal catalysis system for synergistic cancer therapy.Chemical science · 2022Article
- DNA-based enzymatic systems and their applications.iScience · 2022Review
- DNA-based platform for efficient and precisely targeted bioorthogonal catalysis in living systems.Nature communications · 2022Article
- A ruthenium-oligonucleotide bioconjugated photosensitizing aptamer for cancer cell specific photodynamic therapy.RSC chemical biology · 2022Article
- Biosensing with DNAzymes.Chemical Society reviews · 2021Review
- Highly sensitive colorimetric sensing of copper(ii) ions based on "CLICK-17" DNAzyme-catalyzed azide modified gold nanoparticles and alkyne capped dsDNA cycloaddition.RSC advances · 2021Article
- SPAAC-NAD-seq, a sensitive and accurate method to profile NADProceedings of the National Academy of Sciences of the United States of America · 2021Article
- DNAmoreDB, a database of DNAzymes.Nucleic acids research · 2021Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To enable the optimal, biocompatible and non-destructive application of the highly useful copper (Cu+)-mediated alkyne-azide 'click' cycloaddition in water, we have isolated and characterized a 79-nucleotide DNA enzyme or DNAzyme, 'CLICK-17', that harnesses as low as sub-micromolar Cu+; or, surprisingly, Cu2+ (without added reductants such as ascorbate) to catalyze conjugation between a variety of alkyne and azide substrates, including small molecules, proteins and nucleic acids. CLICK-17's Cu+ catalysis is orders of magnitude faster than that of either Cu+ alone or of Cu+ complexed to PERMUT-17, a sequence-permuted DNA isomer of CLICK-17. With the less toxic Cu2+, CLICK-17 attains rates comparable to Cu+, under conditions where both Cu2+ alone and Cu2+ complexed with a classic accelerating ligand, THPTA, are wholly inactive. Cyclic voltammetry shows that CLICK-17, unlike PERMUT-17, powerfully perturbs the Cu(II)/Cu(I) redox potential. CLICK-17 thus provides a unique, DNA-derived ligand environment for catalytic copper within its active site. As a bona fide Cu2+-driven enzyme, with potential for being evolved to accept only designated substrates, CLICK-17 and future variants promise the fast, safe, and substrate-specific catalysis of 'click' bioconjugations, potentially on the surfaces of living cells.
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