Evidence map›Paper›PMID 32520211›Full record

ArticleRevista do Instituto de Medicina Tropical de Sao Paulo2020

The performance of an in-house loop-mediated isothermal amplification for the rapid detection of Mycobacterium tuberculosis in sputum samples in comparison with Xpert MTB/RIF, microscopy and culture.

Benjawan Phetsuksiri, Wiphat Klayut, Janisara Rudeeaneksin, Sopa Srisungngam, Supranee Bunchoo, Sarawut Toonkomdang, Thanee Wongchai, Chie Nakajima, Yasuhiko Suzuki

Open access · diamondAbstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Revista do Instituto de Medicina Tropical de Sao Paulo, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed, 3 pooled it
1.0field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 3 syntheses or guidelines pooled it, 16 citations in OpenAlex.

  1. Pooled it
  2. Revolutionizing diagnosis of pulmonaryFrontiers in microbiology · 2024
    Pooled it
  3. Pooled it
  4. Review and Updates on the Diagnosis of Tuberculosis.Journal of clinical medicine · 2022
    Review
  5. Article
  6. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 2 countries.

Benjawan PhetsuksiriMinistry of Public Health, National Institute of Health, Department of Medical Sciences, Nonthaburi, Thailand.ORCID http://orcid.org/0000-0001-6507-2898
Wiphat KlayutMinistry of Public Health, National Institute of Health, Department of Medical Sciences, Nonthaburi, Thailand.
Janisara RudeeaneksinMinistry of Public Health, National Institute of Health, Department of Medical Sciences, Nonthaburi, Thailand.
Sopa SrisungngamMinistry of Public Health, National Institute of Health, Department of Medical Sciences, Nonthaburi, Thailand.
Supranee BunchooMinistry of Public Health, National Institute of Health, Department of Medical Sciences, Nonthaburi, Thailand.
Sarawut ToonkomdangMae Sot Hospital, Department of Medical Technology, Tak, Thailand.
Thanee WongchaiMae Sot Hospital, Department of Medical Technology, Tak, Thailand.
Chie NakajimaHokkaido University, Research Center for Zoonosis Control, Division of Bioresources, Sapporo, Japan.
Yasuhiko SuzukiHokkaido University, Research Center for Zoonosis Control, Division of Bioresources, Sapporo, Japan.
Ministry of Public Health · THHokkaido University of Science · JPMaesot General Hospital · TH

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Simple, low-cost and effective diagnostic tests for tuberculosis (TB) are needed especially in TB-high burden settings. The present study evaluated the performance of an in-house loop-mediated isothermal amplification (LAMP) for diagnosing TB by comparing it to Xpert MTB/RIF, microscopy and culture. In Thailand, a total of 204 excess sputum samples volume after the processing of cultures were used for Mycobacterium tuberculosis (MTB) detection by Xpert MTB/RIF and LAMP. Based on culture results as the gold standard, the overall sensitivity of LAMP and Xpert MTB/RIF were 82.1% (126/153; 95% confidential interval [CI]: 75.4-88.98%) and 86.9 % (133/153; 95% CI: 80.5-90.8%) respectively, and the specificity of both tests was 100% (51/51; 95% CI: 93.0-100.0%). In comparison with Xpert MTB/RIF, the sensitivity and specificity of LAMP were 94.7% (126/133; 95% CI: 89.5-97.9%), and 100.0% (73/73; 95% CI: 94.9-100.0%), respectively. The average threshold cycle (Ct) of Xpert MTB/RIF detection for positive and negative LAMP results was statistically different, of 18.4 and 27.0, respectively (p < 0.05). In comparison with the acid-fast staining technique, and analyzing LAMP and Xpert MTB/RIF in smear-negative/culture-positive specimens, there was an increase of the detection rate by 47.7% (21/44) and 54.6% (24/44). The diagnostic sensitivity and specificity of LAMP appeared to be comparable to those of Xpert MTB/RIF. We claim that this LAMP has potential to provide a sensitive diagnostic test for the rapid TB diagnosis. It allowed a fast detection of MTB before the cultures and it could be used in resource-limited laboratory settings.

Indexed as

Diagnostic Tests, RoutineDNA, BacterialHumansMolecular Diagnostic TechniquesMycobacterium tuberculosisPolymerase Chain ReactionSensitivity and SpecificitySputumThailandTuberculosisDNA, Bacterial

Identifiers

PMID32520211
PMCPMC7274762
OpenAlexW3033556359

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.