Evidence map›Paper›PMID 32518968›Full record

ArticleMikrochimica acta2020

Development of a fluorescent DNA nanomachine for ultrasensitive detection of Salmonella enteritidis without labeling and enzymes.

Peng Zhang, Mengxiao Song, Linqin Dou, Yuling Xiao, Kaiju Li, Guangzhen Shen, Binwu Ying, Jia Geng, Depo Yang, Zhengzhi Wu

Abstract read
PubMed Publisher
In one paragraph

Article in Mikrochimica acta, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Advances in detection methods for viable Salmonella spp.: current applications and challenges.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2023
    Review
  3. One-Pot Synthesis of Enzyme and Antibody/CaHPOSensors (Basel, Switzerland) · 2023
    Article
  4. Nucleic Acid-Based Nanobiosensor (NAB) Used forNanomaterials (Basel, Switzerland) · 2022
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Peng ZhangSchool of Pharmaceutical Sciences, Guangzhou Higher Education Mega Center, Sun Yat-sen University, Guangzhou, 510006, China.
Mengxiao SongDepartment of Laboratory Medicine, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University and Collaborative Innovation Center for Biotherapy, Chengdu, 610041, China.
Linqin DouDepartment of Laboratory Medicine, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University and Collaborative Innovation Center for Biotherapy, Chengdu, 610041, China.
Yuling XiaoDepartment of Laboratory Medicine, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University and Collaborative Innovation Center for Biotherapy, Chengdu, 610041, China.
Kaiju LiDepartment of Laboratory Medicine, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University and Collaborative Innovation Center for Biotherapy, Chengdu, 610041, China.
Guangzhen ShenSchool of Materials Science and Engineering, Sun Yat-sen University, Guangzhou, 510006, China.
Binwu YingDepartment of Laboratory Medicine, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University and Collaborative Innovation Center for Biotherapy, Chengdu, 610041, China.
Jia GengDepartment of Laboratory Medicine, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University and Collaborative Innovation Center for Biotherapy, Chengdu, 610041, China. geng.jia@scu.edu.cn.ORCID 0000-0003-4753-1039
Depo YangSchool of Pharmaceutical Sciences, Guangzhou Higher Education Mega Center, Sun Yat-sen University, Guangzhou, 510006, China.
Zhengzhi WuShenzhen Institute of Geriatrics, Shenzhen, 518020, China.

Funding

China Postdoctoral Science Foundation 2018M633257National Key Research and Development Program of China 2016YFA0201400National Key Research and Development Program of China 2016YFC1200300Natural Nature Science Foundation of Guangdong Province 2020A1515010995Shenzhen Healthcare Research Project SZBC2018010
6 · The paper itself

Abstract

A capture probe complex containing a specific Salmonella enteritidis (S. enteritidis) aptamer and partly hybridized signal trigger sequence was designed with the ability to directly detect viable S. enteritidis. In the presence of the target S. enteritidis, single-stranded trigger sequences were liberated and in turn reacted with hairpins I, II, and III to initiate the triple strand migration reaction; this in turn produced numerous hairpin I·II·III complexes with scaffolds of copper nanoparticles (CuNPs) and replaced the trigger sequence which initiated the next cycle of triple migration reaction. Cyclically, the reuse of the trigger sequences and the successive, cascading production of scaffolds of CuNPs achieved the synthesis of highly fluorescent CuNPs, thus providing significantly enhanced fluorescent signals to achieve ultrasensitive detection of live S. enteritidis as low as 25 CFU/mL with a linear range of detection from 50 to 10

Indexed as

AnimalsAptamers, NucleotideBiosensing TechniquesCell CountCopperDNADNA ProbesFluorescent DyesFood ContaminationInverted Repeat SequencesLimit of DetectionMetal NanoparticlesNucleic Acid HybridizationPork MeatSalmonella enteritidisSpectrometry, FluorescenceAptamers, NucleotideCopperDNADNA ProbesFluorescent DyesAptamerCopper nanoparticleLabel-freeNanomachineSalmonella enteritidis

Identifiers

PMID32518968

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.