ArticlePlant methods2020
Big data from small tissues: extraction of high-quality RNA for RNA-sequencing from different oilseed
Article in Plant methods, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 13 citations in OpenAlex.
- A dataset of tissue-specific gene expression dynamics during seed development in Brassica.Scientific data · 2025Article
- Article
- Light Intensity-A Key Factor Affecting Flavonoid Content and Expression of Key Enzyme Genes of Flavonoid Synthesis in Tartary Buckwheat.Plants (Basel, Switzerland) · 2022Article
- An optimized method to obtain high-quality RNA from different tissues in Lilium davidii var. unicolor.Scientific reports · 2022Article
- Uncovering Trait Associations Resulting in Maximal Seed Yield in Winter and Spring Oilseed Rape.Frontiers in plant science · 2021Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundObtaining high-quality RNA for gene expression analyses from different seed tissues is challenging due to the presence of various contaminants, such as polyphenols, polysaccharides and lipids which interfere with RNA extraction methods. At present, the available protocols for extracting RNA from seeds require high amounts of tissue and are mainly focused on extracting RNA from whole seeds. However, extracting RNA at the tissue level enables more detailed studies regarding tissue specific transcriptomes during seed development.
resultsSeeds from heart stage embryo to mature developmental stages of
conclusionsHere, we present efficient, reproducible and reliable high-quality RNA extraction methods for diverse oilseed
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Registered trials
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