ArticleScientific reports2020
A novel culture medium with reduced nutrient concentrations supports the development and viability of mouse embryos.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Automated Microvolume Secretome Proteomics Enables Sensitive and Deep Profiling and Noninvasive Single-Embryo Analysis.Journal of proteome research · 2026Article
- Advances in supplementation strategies for enhancing bovine in vitro embryo production: a review of growth factors and antioxidants.Journal of assisted reproduction and genetics · 2025Review
- Altering metabolism programs cell identity via NADThe EMBO journal · 2025Article
- Ovarian Stromal Cell-Conditioned Media, but Not Co-Culture, Improves Survival in Feline Follicles.Animals : an open access journal from MDPI · 2025Article
- Reduction of nutrients concentration in culture medium has no effect on bovine embryo production, pregnancy and birth rates.Scientific reports · 2025Article
- In vitro culture alters cell lineage composition and cellular metabolism of bovine blastocyst†.Biology of reproduction · 2024Article
- The Role of One-Carbon Metabolism and Methyl Donors in Medically Assisted Reproduction: A Narrative Review of the Literature.International journal of molecular sciences · 2024Review
- FOXO1-mediated lipid metabolism maintains mammalian embryos in dormancy.Nature cell biology · 2024Article
- Bovine embryo productionAnimal reproduction · 2024Review
- Lipid Enriched Reduced Nutrient Culture Medium Improves Bovine Blastocyst Formation.Reproduction & fertility · 2023Article
- Estrogen signaling encourages blastocyst development and implantation potential.Journal of assisted reproduction and genetics · 2023Article
- IGF-I Medium Supplementation Improves Singly Cultured Cat Oocyte Maturation and Embryo Development In Vitro.Animals : an open access journal from MDPI · 2021Article
- In Vitro Fertilisation of Mouse Oocytes in L-Proline and L-Pipecolic Acid Improves Subsequent Development.Cells · 2021Article
- Developmental and molecular response of bovine embryos to reduced nutrientsReproduction & fertility · 2020Article
- Short-Term Exposure to Culture Media Used in Human ART Shapes Early Calcium Oscillations in ICSI-Fertilized Mouse Oocytes and Impacts Adult Phenotype.Reproductive medicine and biologyArticle
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Further refinement of culture media is needed to improve the quality of embryos generated in vitro. Previous results from our laboratory demonstrated that uptake of nutrients by the embryo is significantly less than what is supplied in traditional culture media. Our objective was to determine the impact of reduced nutrient concentrations in culture medium on mouse embryo development, metabolism, and quality as a possible platform for next generation medium formulation. Concentrations of carbohydrates, amino acids, and vitamins could be reduced by 50% with no detrimental effects, but blastocyst development was impaired at 25% of standard nutrient provision (reduced nutrient medium; RN). Addition of pyruvate and L-lactate (+PL) to RN at 50% of standard concentrations restored blastocyst development, hatching, and cell number. In addition, blastocysts produced in RN + PL contained more ICM cells and ATP than blastocysts cultured in our control (100% nutrient) medium; however, metabolic activity was altered. Similarly, embryos produced in the RN medium with elevated (50% control) concentrations of pyruvate and lactate in the first step medium and EAA and Glu in the second step medium were competent to implant and develop into fetuses at a similar rate as embryos produced in the control medium. This novel approach to culture medium formulation could help define the optimal nutrient requirements of embryos in culture and provide a means of shifting metabolic activity towards the utilization of specific metabolic pathways that may be beneficial for embryo viability.
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