ArticleNature communications2020
ADP binding by the Culex quinquefasciatus mosquito D7 salivary protein enhances blood feeding on mammals.
Article in Nature communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 34 citations in OpenAlex.
- Chromosome-Level Genome of Simulium vittatum Links Black Fly Cytogenetics to Genome Organization and Evolution.bioRxiv : the preprint server for biology · 2026Article
- Structural genomics sheds light on protein functions and remote homologs across the insect tree of life.Cell research · 2026Article
- Chromosome-scale genome assembly and developmental transcriptome of Aedes triseriatus, vector of La Crosse virus.BMC genomics · 2026Article
- Avian malaria and the overlooked metabolic pathways underlying mosquitoRoyal Society open science · 2025Article
- Mosquito salivary apyrase regulates blood meal hemostasis and facilitates malaria parasite transmission.Nature communications · 2024Article
- Structural and functional comparisons of salivary α-glucosidases from the mosquito vectors Aedes aegypti, Anopheles gambiae, and Culex quinquefasciatus.Insect biochemistry and molecular biology · 2024Article
- The significance of mosquito saliva in arbovirus transmission and pathogenesis in the vertebrate host.One health (Amsterdam, Netherlands) · 2023Article
- Article
- A deeper insight into the sialome of male and female Culex quinquefasciatus mosquitoes.BMC genomics · 2023Article
- The salivary protein Saglin facilitates efficient midgut colonization of Anopheles mosquitoes by malaria parasites.PLoS pathogens · 2023Article
- Mosquito Salivary Proteins and Arbovirus Infection: From Viral Enhancers to Potential Targets for Vaccines.Pathogens (Basel, Switzerland) · 2023Review
- An insight into the female and male Sabethes cyaneus mosquito salivary glands transcriptome.Insect biochemistry and molecular biology · 2023Article
- An Overview of D7 Protein Structure and Physiological Roles in Blood-Feeding Nematocera.Biology · 2022Review
- Performing Immunohistochemistry in Mosquito Salivary Glands.Cold Spring Harbor protocols · 2022Article
- A deeper insight into the sialome of male and female Ochlerotatus triseriatus mosquitoes.Insect biochemistry and molecular biology · 2022Article
- Functional aspects of evolution in a cluster of salivary protein genes from mosquitoes.Insect biochemistry and molecular biology · 2022Article
- Novel salivary antihemostatic activities of long-form D7 proteins from the malaria vector Anopheles gambiae facilitate hematophagy.The Journal of biological chemistry · 2022Article
- Article
- Vertebrate Responses against Arthropod Salivary Proteins and Their Therapeutic Potential.Vaccines · 2021Review
- Article
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
Abstract
During blood-feeding, mosquito saliva is injected into the skin to facilitate blood meal acquisition. D7 proteins are among the most abundant components of the mosquito saliva. Here we report the ligand binding specificity and physiological relevance of two D7 long proteins from Culex quinquefasciatus mosquito, the vector of filaria parasites or West Nile viruses. CxD7L2 binds biogenic amines and eicosanoids. CxD7L1 exhibits high affinity for ADP and ATP, a binding capacity not reported in any D7. We solve the crystal structure of CxD7L1 in complex with ADP to 1.97 Å resolution. The binding pocket lies between the two protein domains, whereas all known D7s bind ligands either within the N- or the C-terminal domains. We demonstrate that these proteins inhibit hemostasis in ex vivo and in vivo experiments. Our results suggest that the ADP-binding function acquired by CxD7L1 evolved to enhance blood-feeding in mammals, where ADP plays a key role in platelet aggregation.
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