ArticleScientific reports2020
Shotgun sequencing of honey DNA can describe honey bee derived environmental signatures and the honey bee hologenome complexity.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed.
- Review
- Honey environmental DNA reveals entomological fingerprints through dual mitochondrial cytochrome c oxidase subunit 1 (COI) and cytochrome b (CYTB) metabarcoding.Scientific reports · 2026Article
- Biogeography from a food matrix: a temporal distribution map of Apis mellifera mitochondrial DNA lineages across Italy, obtained from honey samples.Scientific reports · 2026Article
- The Evolution of Bees: Insights from 'Omic Studies.Genome biology and evolution · 2026Review
- A pilot proof-of-concept study of microbial and botanical diversity in honey samples from Necochea, Argentina.Frontiers in microbiology · 2026Article
- Inferring plant-bee-microbe associations: Foragers, hive workers, and honey tell complementary stories.PloS one · 2026Article
- Distinct colony niches shape associated microbiotas in Neotropical stingless bees.Genetics and molecular biology · 2026Article
- Genome-resolved metagenomics reveals a phylogenetically cohesiveISME communications · 2026Article
- Article
- The Latitudinal Biotic Interaction Hypothesis revisited: contrasting latitudinal richness gradients in actively vs. passively accumulated interaction partners of honey bees.BMC ecology and evolution · 2025Article
- Article
- Honey microbiota, methods for determining the microbiological composition and the antimicrobial effect of honey - A review.Food chemistry: X · 2024Review
- Molecular Detection and Phylogenetic Relationships of Honey Bee-Associated Viruses in Bee Products.Veterinary sciences · 2024Article
- Tracking the hologenome dynamics in aquatic invertebrates by the holo-2bRAD approach.Communications biology · 2024Article
- Using pollen DNA metabarcoding to trace the geographical and botanical origin of honey from Karangasem, Indonesia.Heliyon · 2024Article
- An innovative molecular approach towards the cost-effective entomological authentication of honey.NPJ science of food · 2024Article
- The buzz about honey-based biosurveys.npj biodiversity · 2024Review
- Shotgun Metagenomics Reveals Minor Micro"International journal of molecular sciences · 2024Article
- A preliminary metabarcoding analysis of Portuguese raw honeys.Archives of microbiology · 2023Article
- The role of seasonality in shaping the interactions of honeybees with other taxa.Ecology and evolution · 2023Article
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5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Honey bees are large-scale monitoring tools due to their extensive environmental exploration. In their activities and from the hive ecosystem complex, they get in close contact with many organisms whose traces can be transferred into the honey, which can represent an interesting reservoir of environmental DNA (eDNA) signatures and information useful to analyse the honey bee hologenome complexity. In this study, we tested a deep shotgun sequencing approach of honey DNA coupled with a specifically adapted bioinformatic pipeline. This methodology was applied to a few honey samples pointing out DNA sequences from 191 organisms spanning different kingdoms or phyla (viruses, bacteria, plants, fungi, protozoans, arthropods, mammals). Bacteria included the largest number of species. These multi-kingdom signatures listed common hive and honey bee gut microorganisms, honey bee pathogens, parasites and pests, which resembled a complex interplay that might provide a general picture of the honey bee pathosphere. Based on the Apis mellifera filamentous virus genome diversity (the most abundant detected DNA source) we obtained information that could define the origin of the honey at the apiary level. Mining Apis mellifera sequences made it possible to identify the honey bee subspecies both at the mitochondrial and nuclear genome levels.
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