ArticlemBio2020
Critical Relevance of Stochastic Effects on Low-Bacterial-Biomass 16S rRNA Gene Analysis.
Article in mBio, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT03034642 (Modulation of Steroid Immunosuppression by Alveolar Efferocytosis), which is not on this map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Modulation of Steroid Immunosuppression by Alveolar Efferocytosis
Who cites it
29 citing papers in PubMed.
- Methodological bias shapes the interpretation of the urinary microbiome in low-biomass systems.Frontiers in microbiology · 2026Review
- Sampling method influences hemolymph bacterial communities in the eastern oyster,Frontiers in microbiology · 2026Article
- Microbial contribution to metabolic niche formation varies across the respiratory tract.Cell host & microbe · 2025Article
- Taxonomic Diversity and Clinical Correlations in Periapical Lesions by Next-Generation Sequencing Analysis.Genes · 2025Article
- Microbial biomarker development for detection and prognosis of early-stage non-small cell lung cancer.Cancer biomarkers : section A of Disease markers · 2025Review
- Intratumoral microbiota-aided fusion radiomics model for predicting tumor response to neoadjuvant chemoimmunotherapy in triple-negative breast cancer.Journal of translational medicine · 2025Article
- De-biasing microbiome sequencing data: bacterial morphology-based correction of extraction bias and correlates of chimera formation.Microbiome · 2025Article
- Metagenomic sequencing of CRISPRs as a new marker to aid in personal identification with low-biomass samples.mSystems · 2024Article
- Long journey of 16S rRNA-amplicon sequencing toward cell-based functional bacterial microbiota characterization.iMetaOmics · 2024Review
- Tissue-resident bacteria in metabolic diseases: emerging evidence and challenges.Nature metabolism · 2024Review
- Emerging clinical relevance of microbiome in cancer: promising biomarkers and therapeutic targets.Protein & cell · 2024Review
- Azithromycin alters spatial and temporal dynamics of airway microbiota in idiopathic pulmonary fibrosis.ERJ open research · 2023Article
- The dynamic lung microbiome in health and disease.Nature reviews. Microbiology · 2023Review
- Is there a placental microbiota? A critical review and re-analysis of published placental microbiota datasets.BMC microbiology · 2023Review
- Article
- Technical challenges regarding the use of formalin-fixed paraffin embedded (FFPE) tissue specimens for the detection of bacterial alterations in colorectal cancer.BMC microbiology · 2021Article
- Insights into epidemiologic assessments of the microbiome and challenges in identifying microbiome relationships with adverse pregnancy outcomes.Current epidemiology reports · 2021Article
- The lung microbiome: progress and promise.The Journal of clinical investigation · 2021Review
- Toll-like receptors, environmental caging, and lung dysbiosis.American journal of physiology. Lung cellular and molecular physiology · 2021Article
- Exploring the Individual Bacterial Microbiota of QuestingMicroorganisms · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
14 authors.
Funding
Abstract
The bacterial microbiome of human body sites, previously considered sterile, remains highly controversial because it can be challenging to isolate signal from noise when low-biomass samples are being analyzed. We tested the hypothesis that stochastic sequencing noise, separable from reagent contamination, is generated during sequencing on the Illumina MiSeq platform when DNA input is below a critical threshold. We first purified DNA from serial dilutions of
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.