ArticleAnais brasileiros de dermatologia
Identification of Leishmania species by high-resolution DNA dissociation in cases of American cutaneous leishmaniasis.
Article in Anais brasileiros de dermatologia. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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8 citing papers in PubMed, 14 citations in OpenAlex.
- Easy Identification ofACS omega · 2025Article
- Comparison of Sampling Procedures for the Molecular Diagnosis of Leishmaniases.The American journal of tropical medicine and hygiene · 2023Article
- Antileishmanial Activity ofPlants (Basel, Switzerland) · 2023Article
- Comparison between Colorimetric In Situ Hybridization, Histopathology, and Immunohistochemistry for the Diagnosis of New World Cutaneous Leishmaniasis in Human Skin Samples.Tropical medicine and infectious disease · 2022Article
- Determinants of cutaneous leishmaniasis among students in Delanta district, Northeast Ethiopia: A case-control study.Health science reports · 2022Article
- Knowledge, attitude and prevention measures of students towards cutaneous leishmaniasis in Delanta district, Northeast Ethiopia.Parasite epidemiology and control · 2022Article
- Epidemiological and diagnostic aspects of feline leishmaniasis with emphasis on Brazil: a narrative review.Parasitology research · 2022Review
- Development of an Amplicon-Based Next-Generation Sequencing Protocol to IdentifyMicrobiology spectrum · 2021Article
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5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAmerican cutaneous leishmaniasis is an infectious dermatosis caused by protozoa of the genus Leishmania, which comprises a broad spectrum of clinical manifestations depending on the parasite species involved in the infections and the immunogenetic response of the host. The use of techniques for amplification of the parasites DNA based on polymerase chain reaction polymerase chain reaction and the recent application of combined techniques, such as high-resolution DNA dissociation, have been described as a viable alternative for the detection and identification of Leishmania spp. in biological samples.
objectivesTo identify the Leishmania species using the polymerase chain reaction high-resolution DNA dissociation technique in skin biopsies of hospital-treated patients, and compare with results obtained by other molecular identification techniques.
methodsA retrospective study assessing patients with suspected American cutaneous leishmaniasis seen at a hospital in São Paulo/Brazil was conducted. The paraffin blocks of 22 patients were analyzed by polymerase chain reaction high-resolution DNA dissociation to confirm the diagnosis and identify the species.
resultsOf the 22 patients with suspected American cutaneous leishmaniasis, the parasite was identified in 14, comprising five cases (35.6%) of infection by L. amazonensis, four (28.5%) by L. braziliensis, two (14.4%) by L. amazonensis+L. infantum chagasi, two (14.4%) by L. guyanensis, and one (7.1%) by Leishmania infantum chagasi. In one of the samples, in which the presence of amastigotes was confirmed on histopathological examination, the polymerase chain reaction high-resolution DNA dissociation technique failed to detect the DNA of the parasite. STUDY LIMITATIONS: The retrospective nature of the study and small number of patients.
conclusionsThe method detected and identified Leishmania species in paraffin-embedded skin biopsies with a sensitivity of 96.4% and could be routinely used in the public health system.
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