ArticleStem cell research & therapy2020
Comparison of senescence-related changes between three- and two-dimensional cultured adipose-derived mesenchymal stem cells.
Article in Stem cell research & therapy, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed.
- Article
- OCT4-driven PER1 activation promotes odontogenic differentiation in senescent SCAP spheres.International journal of oral science · 2026Article
- Time-restricted feeding improves functional capacity of adipose-derived stem cells with activation of OSK-associated transcriptional programs.npj aging · 2026Article
- The correlation between telomere length of the peripheral blood basophil and the risk and prognosis of hepatocellular carcinoma.Discover oncology · 2026Article
- Free-floating long-term vascularized mesenchymal organoids.iScience · 2026Article
- Radiation-Induced EMT of Adipose-Derived Stem Cells in 3D Organotypic Culture via Notch Signaling Pathway.Biology · 2025Article
- Matrix Viscoelasticity Orchestrates Osteogenesis via Mechanotransduction Mediated Metabolic Switch in Macrophages.Advanced healthcare materials · 2025Article
- Inhibition of GSK-3β Restores Differentiation Potential of Late-Passage Mesenchymal Stem Cells.Pharmaceuticals (Basel, Switzerland) · 2025Article
- Effects of simulated microgravity on dental pulp stem cell stemness.Journal of molecular histology · 2025Article
- High-yield BMSC-derived exosomes by the 3D culture system to enhance the skin wound repair.Regenerative biomaterials · 2025Article
- Small adipose-derived mesenchymal stromal cells exhibit longer telomeres and enhanced regenerative potential.Frontiers in bioengineering and biotechnology · 2025Article
- 3D culture inhibits replicative senescence of SCAPs via UQCRC2-mediated mitochondrial oxidative phosphorylation.Journal of translational medicine · 2024Article
- Scaffold-free 3D culture systems for stem cell-based tissue regeneration.APL bioengineering · 2024Review
- Therapeutic effect of three-dimensional hanging drop cultured human umbilical cord mesenchymal stem cells on osteoarthritis in rabbits.Stem cell research & therapy · 2024Article
- Senescence in Adipose-Derived Stem Cells: Biological Mechanisms and Therapeutic Challenges.International journal of molecular sciences · 2024Review
- Characterization of 3D Organotypic Culture of Mouse Adipose-Derived Stem Cells.International journal of molecular sciences · 2024Article
- Advantages of Using 3D Spheroid Culture Systems in Toxicological and Pharmacological Assessment for Osteogenesis Research.International journal of molecular sciences · 2024Review
- Sestrin1, 2, and 3 are dispensable for female fertility in mice.Journal of ovarian research · 2024Article
- Insights on Three Dimensional Organoid Studies for Stem Cell Therapy in Regenerative Medicine.Stem cell reviews and reports · 2024Review
- Advancements in culture technology of adipose-derived stromal/stem cells: implications for diabetes and its complications.Frontiers in endocrinology · 2024Review
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Authors and funding
11 authors.
Funding
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Abstract
backgroundAdipose-derived mesenchymal stem cells (ADMSCs) have attracted widespread interest as cell-based tissue repair systems. To obtain adequate quantities of ADMSCs for therapeutic applications, extensive in vitro expansion is required. However, under current two-dimensional (2D) approaches, ADMSCs rapidly undergo replicative senescence, and cell growth is impeded and stem cell properties are eliminated by mechanisms that are poorly understood. These issues limit the extensive applications of ADMSCs. In this study, we investigated senescence-related changes in mesenchymal stem cells (MSCs) isolated from human adipose tissue in 2D and three-dimensional (3D) cultures.
methodsWe studied cell growth over a given period (21 days) to determine if modes of culture were associated with ADMSC senescence. ADMSCs were isolated from healthy females by liposuction surgery and then were grown in 2D and 3D cultures. The cell morphology was observed during cell culture. Every other time of culture, senescence-associated β-galactosidase (SA-β-gal) expression, cell viability, proliferation, and differentiation potential of ADMSCs from 2D and 3D cultures were detected. Also, senescence- and stemness-related gene expression, telomere length, telomerase activity, and energy metabolism of ADMSCs for different culture times were evaluated.
resultsWith long-term propagation, we observed significant changes in cell morphology, proliferation, differentiation abilities, and energy metabolism, which were associated with increases in SA-β-gal activity and decreases in telomere length and telomerase activity. Notably, when cultured in 3D, these changes were improved.
conclusionsOur results indicate that 3D culture is able to ameliorate senescence-related changes in ADMSCs.
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