ArticleBMC pregnancy and childbirth2020
Development and analytical validation of real-time PCR for the detection of Streptococcus agalactiae in pregnant women.
Article in BMC pregnancy and childbirth, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Development of a LAMP assay using hybridization-based TaqMan-style probes (HyTaq) for simultaneous detection of wild-type and macrolide-resistantJournal of clinical microbiology · 2026Article
- Comparison of qPCR and chromogenic culture methods for rapid detection of group B streptococcus colonization in Vietnamese pregnant women.Practical laboratory medicine · 2024Article
- Development and performance evaluation of a qPCR-based assay for the fully automated detection of group BMicrobiology spectrum · 2024Article
- Development of a multiplex Loop-Mediated Isothermal Amplification (LAMP) for the diagnosis of bacterial periprosthetic joint infection.PloS one · 2024Article
- Ultra-Fast Impedimetric Immunoassay for Detection ofMicromachines · 2023Article
- An Application of Real-Time PCR and CDC Protocol May Significantly Reduce the Incidence ofPathogens (Basel, Switzerland) · 2022Article
- Development and Validation of Two Diagnostic Real-Time PCR (TaqMan) Assays for the Detection ofMicroorganisms · 2021Article
Corrections and comments
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Authors and funding
13 authors.
Funding
Abstract
backgroundGroup B Streptococcus (GBS) is the leading cause of invasive neonatal infection. In this study, we aimed to evaluate the analytical validation of qualitative real-time polymerase chain reaction (qPCR) as a means to detect GBS.
methodsGenomic DNA (gDNA) was purified from 12 ATCC bacterial strains, two belonging to GBS and the remainder acting as negative controls. Additionally, gDNA was isolated from 21 strains of GBS from various serotypes (Ia, Ib and II-VIII). All gDNA was used to evaluate the analytical validation of the qPCR method employing a specific Taqman probe. Inclusivity, exclusivity, anticipated reportable range, the limit of detection and robustness were evaluated. The methods used are described in international guidelines and other existing reports. The performance of this qPCR method for detecting GBS was compared to other microbiological methods used with vaginal-rectal samples from pregnant women.
resultsOur qPCR method for detecting GBS was analytically validated. It has a limit of detection of 0.7 GE/μL and 100% analytical specificity. It detects all strains of GBS with the same level of performance as microbiological methods.
conclusionData suggest that this qPCR method performs adequately as a means to detect GBS in vaginal-rectal swabs from pregnant women.
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