ArticleJournal of clinical virology : the official publication of the Pan American Society for Clinical Virology2020
Evaluation of the commercially available LightMix® Modular E-gene kit using clinical and proficiency testing specimens for SARS-CoV-2 detection.
Article in Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 57 citations in OpenAlex.
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- Comparison of diagnostic performance of five molecular assays for detection of SARS-CoV-2.Diagnostic microbiology and infectious disease · 2021Article
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- Optimizing testing regimes for the detection of COVID-19 in children and older adults.Expert review of molecular diagnostics · 2021Review
- Comparison of eleven in vitro diagnostic assays for the detection of SARS-CoV-2 RNA.Journal of virological methods · 2021Article
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- Comparative evaluation of a dual-target real-time RT-PCR assay for COVID-19 diagnosis and assessment of performance in pooled saliva and nasopharyngeal swab samples.Expert review of molecular diagnostics · 2021Article
- Minimally Invasive Autopsy Practice in COVID-19 Cases: Biosafety and Findings.Pathogens (Basel, Switzerland) · 2021Article
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Corrections and comments
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Authors and funding
18 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRapid and sensitive diagnostic assays for SARS-CoV-2 detection are required for prompt patient management and infection control. The analytical and clinical performances of LightMix® Modular SARS and Wuhan CoV E-gene kit, a widely used commercial assay for SARS-CoV-2 detection, have not been well studied.
objectiveTo evaluate the performance characteristics of the LightMix® E-gene kit in comparison with well-validated in-house developed COVID-19 RT-PCR assays. STUDY
designSerial dilutions of SARS-CoV-2 culture isolate extracts were used for analytical sensitivity evaluation. A total of 289 clinical specimens from 186 patients with suspected COVID-19 and 8 proficiency testing (PT) samples were used to evaluate the diagnostic performance of the LightMix® E-gene kit against in-house developed COVID-19-RdRp/Hel and COVID-19-N RT-PCR assays.
resultsThe LightMix® E-gene kit had a limit of detection of 1.8 × 10
conclusionsThe LightMix® E-gene kit is a rapid and sensitive assay for SARS-CoV-2 detection. It has fewer verification requirements compared to laboratory-developed tests.
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