Evidence map›Paper›PMID 32516576›Full record

ArticleCell metabolism2020

Toll-Like Receptors Induce Signal-Specific Reprogramming of the Macrophage Lipidome.

Wei-Yuan Hsieh, Quan D Zhou, Autumn G York, Kevin J Williams, Philip O Scumpia, Eliza B Kronenberger, Xen Ping Hoi, Baolong Su, Xun Chi, Viet L Bui and 8 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell metabolism, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 99 papers.

0numbers the graph read from it
0cells of the map it votes in
99citing papers in PubMed
6.3field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

99 citing papers in PubMed, 154 citations in OpenAlex.

  1. Review
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  3. Design and structural basis of selective nonsteroidal inhibitors of aster cholesterol trafficking.Proceedings of the National Academy of Sciences of the United States of America · 2026
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39 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors at 5 institutions in 1 country.

Wei-Yuan HsiehDepartment of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, CA 90095, USA.
Quan D ZhouDepartment of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA.
Autumn G YorkDepartment of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, CA 90095, USA; Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA; Department of Immunobiology, Yale University School of Medicine, New Haven, CT 06520, USA; Howard Hughes Medical Institute, Yale University, New Haven, CT 06520, USA.
Kevin J WilliamsDepartment of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, CA 90095, USA.
Philip O ScumpiaDepartment of Medicine, Division of Dermatology, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA.
Eliza B KronenbergerDepartment of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, CA 90095, USA.
Xen Ping HoiDepartment of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, CA 90095, USA.
Baolong SuDepartment of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, CA 90095, USA.
Xun ChiDepartment of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, CA 90095, USA.
Viet L BuiDepartment of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, CA 90095, USA; Division of Rheumatology, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA.
Elvira KhialeevaMolecular Biology Institute, University of California, Los Angeles, Los Angeles, CA 90095, USA.
Amber KaplanDepartment of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, CA 90095, USA.
Young Min SonDepartment of Immunology, Mayo Clinic Alix School of Medicine, Rochester, MN 55905, USA; Division of Pulmonary and Critical Care Medicine, Department of Medicine, Mayo Clinic Alix School of Medicine, Rochester, MN 55905, USA.
Ajit S DivakaruniDepartment of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA.
Jie SunDepartment of Immunology, Mayo Clinic Alix School of Medicine, Rochester, MN 55905, USA; Division of Pulmonary and Critical Care Medicine, Department of Medicine, Mayo Clinic Alix School of Medicine, Rochester, MN 55905, USA.
Stephen T SmaleDepartment of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, CA 90095, USA; Molecular Biology Institute, University of California, Los Angeles, Los Angeles, CA 90095, USA.
Richard A FlavellDepartment of Immunobiology, Yale University School of Medicine, New Haven, CT 06520, USA; Howard Hughes Medical Institute, Yale University, New Haven, CT 06520, USA.
Steven J BensingerDepartment of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, CA 90095, USA; Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA. Electronic address: sbensinger@mednet.ucla.edu.
University of California, Los Angeles · USHoward Hughes Medical Institute · USMayo Clinic · USMayo Clinic in Arizona · USYale University · US

Funding

Women's CancersP30CA016042 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Samuel Wheeler French · 1985 to 2026
$134.5M
UCLA Clinical Translational Science InstituteUL1TR001881 · NCATS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI ARLEEN F. BROWN, ARASH NAEIM · 2016 to 2026
$118.1M
Yale Clinical and Translational Science Award (U Component)UL1TR001863 · NCATS · YALE UNIVERSITY · PI John H. Krystal, LUCILA OHNO-MACHADO · 2016 to 2026
$102.9M
Understanding the Influence of Lipid Homeostasis on T cell FunctionP01HL146358 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI FONG, LOREN GI · 2019 to 2023
$11.7M
Regulation of pulmonary anti-viral immunity in normal and obese hostsR01HL126647 · NHLBI · MAYO CLINIC ROCHESTER · PI SUN, JIE · 2015 to 2019
$1.9M
Understanding the influence of SREBP signaling on CD4 T helper cell biologyR01AI122282 · NIAID · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI BENSINGER, STEVEN J · 2016 to 2020
$1.9M
Simultaneously boosting both humoral and cellular immunity following vaccinationR01AG047156 · NIA · MAYO CLINIC ROCHESTER · PI SUN, JIE · 2015 to 2019
$1.6M
Determining the Impact of SREBP on Adaptive ImmunityR01AI093768 · NIAID · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI BENSINGER, STEVEN J · 2012 to 2015
$1.5M
Regulation of macrophage transcriptional networks by stress pathways in the skinK08AR066545 · NIAMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI SCUMPIA, PHILIP · 2014 to 2018
$781k
Cytosolic DNA sensors in cutaneous wound healing and host defenseR03AR073940 · NIAMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI SCUMPIA, PHILIP · 2018 to 2019
$156k
Howard Hughes Medical InstituteNCATS NIH HHS UL1 TR001863NCATS NIH HHS UL1 TR001881NCI NIH HHS P30 CA016042NHLBI NIH HHS P01 HL146358NHLBI NIH HHS R01 HL126647NIAID NIH HHS R01 AI093768NIAID NIH HHS R01 AI122282NIAMS NIH HHS K08 AR066545NIAMS NIH HHS R03 AR073940NIA NIH HHS R01 AG047156
6 · The paper itself

Abstract

Macrophages reprogram their lipid metabolism in response to activation signals. However, a systems-level understanding of how different pro-inflammatory stimuli reshape the macrophage lipidome is lacking. Here, we use complementary "shotgun" and isotope tracer mass spectrometry approaches to define the changes in lipid biosynthesis, import, and composition of macrophages induced by various Toll-like receptors (TLRs) and inflammatory cytokines. "Shotgun" lipidomics data revealed that different TLRs and cytokines induce macrophages to acquire distinct lipidomes, indicating their specificity in reshaping lipid composition. Mechanistic studies showed that differential reprogramming of lipid composition is mediated by the opposing effects of MyD88- and TRIF-interferon-signaling pathways. Finally, we applied these insights to show that perturbing reprogramming of lipid composition can enhance inflammation and promote host defense to bacterial challenge. These studies provide a framework for understanding how inflammatory stimuli reprogram lipid composition of macrophages while providing a knowledge platform to exploit differential lipidomics to influence immunity.

Indexed as

LipidomicsAnimalsCell LineMacrophagesMaleMiceMice, KnockoutMice, TransgenicSignal TransductionToll-Like ReceptorsToll-Like Receptorsacetylated-LDLhost defenseinflammationinterferonlipidomicsmacrophagesMyD88stable isotope tracer analysisstearoyl-CoA desaturasetoll-like receptors

Identifiers

PMID32516576
PMCPMC7891175
OpenAlexW3033146331

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.