ArticlePlant biotechnology journal2020
Overexpression of improved EPSPS gene results in field level glyphosate tolerance and higher grain yield in rice.
Article in Plant biotechnology journal, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 76 citations in OpenAlex.
- Engineering glyphosate tolerance in sugarcane through overexpression of a mutated version of its nativeGM crops & food · 2026Article
- Breeding of glyphosate-tolerant genetically modified rice BriA15-38.GM crops & food · 2026Article
- OsEPSPS Balances Disease Resistance and Plant Growth.Plant, cell & environment · 2026Article
- Herbicide Resistance Genes in Crops: Mechanisms, Progress, and Future Perspectives.Plants (Basel, Switzerland) · 2026Review
- Genetically Modified Plants in Agriculture.Biology · 2026Review
- Host-Induced Silencing of Rhizoctonia Solani 5-Enolpyruvylshikimate-3-Phosphate Synthase Impairs Its Virulence in Rice.Plant biotechnology journal · 2026Article
- Development of dual base editors based on ssDNA-targeting SCP1.201 deaminases for the artificial evolution of novel herbicide-tolerant OsEPSPS variants.Plant communications · 2026Article
- Development of an ultra-efficient prime editing system in tomato.Nature communications · 2026Article
- Continued overexpression ofFrontiers in plant science · 2026Article
- Analysis of Photosynthetic Parameters, Yield, and Quality Correlations in Herbicide-Tolerant Transgenic Hybrid Cotton.International journal of molecular sciences · 2025Article
- Evolution and Future Directions in Herbicide-Resistant Crop Development and Weed Management.Rice (New York, N.Y.) · 2025Review
- Methods Established forPlants (Basel, Switzerland) · 2025Article
- Breeding herbicide-resistant rice using CRISPR-Cas gene editing and other technologies.Plant communications · 2025Review
- A Gemini Virus-Derived Autonomously Replicating System for HDR-Mediated Genome Editing of thePlants (Basel, Switzerland) · 2025Article
- Prime editing using paired pegRNAs targeting NG- or NGG-PAM in rice.Frontiers in genome editing · 2025Article
- Exploring the potential role of EPSPS mutations for enhanced glyphosate resistance inFrontiers in plant science · 2025Article
- Affecting of Glyphosate Tolerance and Metabolite Content in TransgenicPlants (Basel, Switzerland) · 2024Article
- Evolving dual-trait EPSP synthase variants using a synthetic yeast selection system.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Heat-stress-responsive HvHSFA2e gene regulates the heat and drought tolerance in barley through modulation of phytohormone and secondary metabolic pathways.Plant cell reports · 2024Article
- Synthetic directed evolution for targeted engineering of plant traits.Frontiers in plant science · 2024Review
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Authors and funding
12 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Glyphosate is a popular, systemic, broad-spectrum herbicide used in modern agriculture. Being a structural analog of phosphoenolpyruvate (PEP), it inhibits 5-enolpyruvylshikimate 3-phosphate synthase (EPSPS) which is responsible for the biosynthesis of aromatic amino acids and various aromatic secondary metabolites. Taking a lead from glyphosate-resistant weeds, two mutant variants of the rice EPSPS gene were developed by amino acid substitution (T173I + P177S; TIPS-OsEPSPS and G172A + T173I + P177S; GATIPS-OsEPSPS). These mutated EPSPS genes were overexpressed in rice under the control of either native EPSPS or constitutive promoters (maize ubiquitin [ZmUbi] promoter). The overexpression of TIPS-OsEPSPS under the control of the ZmUbi promoter resulted in higher tolerance to glyphosate (up to threefold of the recommended dose) without affecting the fitness and related agronomic traits of plants in both controlled and field conditions. Furthermore, such rice lines produced 17%-19% more grains compared to the wild type (WT) in the absence of glyphosate application and the phenylalanine and tryptophan contents in the transgenic seeds were found to be significantly higher in comparison with WT seeds. Our results also revealed that the native promoter guided expression of modified EPSPS genes did not significantly improve the glyphosate tolerance. The present study describing the introduction of a crop-specific TIPS mutation in class I aroA gene of rice and its overexpression have potential to substantially improve the yield and field level glyphosate tolerance in rice. This is the first report to observe that the EPSPS has role to play in improving grain yield of rice.
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