Evidence map›Paper›PMID 32516446›Full record

ArticleParasite immunology2020

Human immunoglobulin G responses to Cimex lectularius L. saliva.

Johnathan M Sheele, Brian Ferrari, Jerome Goddard, Danie Schlatzer, Kathleen C Lundberg, Katirina Guinto, Monica E Embers, Andrew B Young, Gale E Ridge, Giovanni Damiani and 1 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Parasite immunology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.1field-weighted citation impact, top 49% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 3 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 5 institutions in 1 country.

Johnathan M SheeleDepartment of Emergency Medicine, Mayo Clinic, Jacksonville, Florida, USA.ORCID 0000-0003-4503-8590
Brian FerrariImmune Function Core Facility, Case Western Reserve University, Cleveland, Ohio, USA.
Jerome GoddardDepartment of Biochemistry, Molecular Biology, Entomology, and Plant Pathology, Mississippi State University, Starkville, Mississippi, USA.
Danie SchlatzerCenter for Proteomics and Bioinformatics, Case Western Reserve University, Cleveland, Ohio, USA.
Kathleen C LundbergCenter for Proteomics and Bioinformatics, Case Western Reserve University, Cleveland, Ohio, USA.
Katirina GuintoCase Western Reserve University, Cleveland, Ohio, USA.
Monica E EmbersDivision of Immunology, Tulane University National Primate Research Center, Covington, Louisiana, USA.
Andrew B YoungDepartment of Dermatology, Case Western Reserve University, Cleveland, Ohio, USA.
Gale E RidgeThe Connecticut Agricultural Experiment Station, New Haven, Connecticut, USA.
Giovanni DamianiDepartment of Dermatology, Case Western Reserve University, Cleveland, Ohio, USA.
Thomas S McCormickDepartment of Dermatology, Case Western Reserve University, Cleveland, Ohio, USA.
Case Western Reserve University · USConnecticut Agricultural Experiment Station · USJacksonville College · USMississippi State University · USTulane University · US

Funding

Tulane NPRC SPF Sheltered Outdoor Enclosure ExpansionP51OD011104 · OD · TULANE UNIVERSITY OF LOUISIANA · PI L Lee HAMM · 2012 to 2026
$142.4M
Clinical and Translational Science Collaborative of ClevelandUL1TR000439 · NCATS · CASE WESTERN RESERVE UNIVERSITY · PI KONSTAN, MICHAEL W. · 2012 to 2016
$50.0M
Clinical and Translational Science Collaborative of ClevelandUL1TR002548 · NCATS · CASE WESTERN RESERVE UNIVERSITY · PI MCCOMSEY, GRACE A · 2018 to 2022
$35.5M
NCATS NIH HHS TR000439NCATS NIH HHS UL1 TR000439NCATS NIH HHS UL1 TR002548NIH HHS P51 OD011104
6 · The paper itself

Abstract

aimsTo investigate the immunoglobulin (Ig) G response after being fed upon by Cimex lectularius L. METHODS AND

resultsParticipants were fed upon by three male C lectularius insects weekly for a month. Blood was obtained before the feeding and at the last feeding, which was used for immunoblots against bed bug salivary gland extract, with antihuman Immunoglobulin G (IgG) secondary antibodies. No consistent IgG changes developed in 11 humans serially fed upon by C lectularius. Two participants had new IgG responses to proteins at molecular weights of approximately 12-13 kDa, and one had an IgG response to a protein at approximately 40 kDa. At the last study visit, more intense IgG bands to proteins at molecular weights of 12-13 kDa had developed in 55% of participants (6/11) and at molecular weights of ≈30, ≈40 and ≈70 kDa in 45% (5/11) compared with the first study visit. Nitrophorin and apyrase were the most common C lectularius proteins identified with liquid chromatography-tandem mass spectrometry in both crushed bed bug salivary gland extract and post-bed bug feeding extract.

conclusionsHuman participants did not have consistent IgG responses to crushed C lectularius salivary gland extract.

Indexed as

AdolescentAdultAnimalsBedbugsFemaleHumansImmunoglobulin GInsect Bites and StingsMaleMiddle AgedSalivaSalivary GlandsSalivary Proteins and PeptidesYoung AdultImmunoglobulin GSalivary Proteins and Peptidesantibodybed bugCimex lectulariuscimicosisIgGimmunoglobulinsaliva

Identifiers

PMID32516446
PMCPMC7668222
OpenAlexW3033852282

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.