Evidence map›Paper›PMID 32515871›Full record

ArticleCurrent protocols in microbiology2020

Giardia lamblia: Laboratory Maintenance, Lifecycle Induction, and Infection of Murine Models.

Marc Y Fink, Danielle Shapiro, Steven M Singer

Abstract read
In one paragraph

Article in Current protocols in microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed.

  1. Article
  2. Analysis ofInfection and immunity · 2026
    Article
  3. Article
  4. Parasitology · 2026
    Review
  5. Antiparasitic Activity ofVeterinary sciences · 2026
    Article
  6. Article
  7. Article
  8. Review
  9. Review
  10. Article
  11. Article
  12. Review
  13. bioRxiv : the preprint server for biology · 2024
    Article
  14. Article
  15. Review
  16. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Marc Y FinkDepartment of Biology, Georgetown University, Washington, District of Columbia.
Danielle ShapiroDepartment of Biology, Georgetown University, Washington, District of Columbia.
Steven M SingerDepartment of Biology, Georgetown University, Washington, District of Columbia.

Funding

The Role of Macrophages in Immune Responses to GiardiaR15AI109591 · NIAID · GEORGETOWN UNIVERSITY · PI SINGER, STEVEN M · 2014 to 2023
$1.5M
Immune Control of Giardia PathogenesisR15AI094492 · NIAID · GEORGETOWN UNIVERSITY · PI SINGER, STEVEN M · 2011 to 2011
$461k
NIAID NIH HHS R15 AI094492NIAID NIH HHS R15 AI109591
6 · The paper itself

Abstract

Giardia lamblia is a protozoan parasite that is found ubiquitously throughout the world and is a major contributor to diarrheal disease. Giardia exhibits a biphasic lifestyle existing as either a dormant cyst or a vegetative trophozoite. Infections are typically initiated through the consumption of cyst-contaminated water or food. Giardia was first axenized in the 1970s and can be readily maintained in a laboratory setting. Additionally, Giardia is one of the few protozoans that can be induced to complete its complete lifecycle using laboratory methods. In this article, we outline protocols to maintain Giardia and induce passage through its lifecycle. We also provide protocols for infecting and quantifying parasites in an animal infection model. © 2020 Wiley Periodicals LLC. Basic Protocol 1: In vitro maintenance and growth of Giardia trophozoites Basic Protocol 2: In vitro encystation of Giardia cysts Basic Protocol 3: In vivo infections using Giardia trophozoites.

Indexed as

AnimalsCell Culture TechniquesDisease Models, AnimalGiardia lambliaGiardiasisHumansLife Cycle StagesMiceParasitologyPreservation, BiologicalProtozoan ProteinsTrophozoitesProtozoan Proteinsanimal modelcryopreservationGiardia lambliagiardiasismurine infectionpassage and maintenanceprotozoan parasite

Identifiers

PMID32515871
PMCPMC7339241

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.