ArticleChemistry (Weinheim an der Bergstrasse, Germany)2020
The Diversity of a Polyclonal FluCell-SELEX Library Outperforms Individual Aptamers as Emerging Diagnostic Tools for the Identification of Carbapenem Resistant Pseudomonas aeruginosa.
Article in Chemistry (Weinheim an der Bergstrasse, Germany), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 28 citations in OpenAlex.
- Single-sequence based gFET-aptasensors for the discrimination of apo- and holo-RBP4 in human serum.Scientific reports · 2026Article
- Aptamer Engineering: Strategies for Discovering Functional Nucleic Acids for Next-Generation Diagnostics and Biosensing.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- An Aptamer-Based gFET-Sensor for Specific Quantification of Gene Therapeutic Human Adenovirus Type 5.Biosensors · 2025Article
- The Power of Old Hats: Rediscovering Inosine-EpPCR to Create Starting Libraries for Whole-Cell-SELEX.Biosensors · 2025Article
- Specific gFET-Based Aptasensors for Monitoring of Microbiome Quality: Quantification of the Enteric Health-Relevant Bacterium Roseburia Intestinalis.Advanced healthcare materials · 2025Article
- IMPATIENT-qPCR: monitoring SELEX success during in vitro aptamer evolution.Applied microbiology and biotechnology · 2024Article
- Robust Fluorometric Aptamer Assay for Direct and Rapid Detection of Clinical Isolates ofInternational journal of molecular sciences · 2024Article
- Enriched Aptamer Libraries in Fluorescence-Based Assays forMicroorganisms · 2023Article
- Aptamers as Novel Binding Molecules on an Antimicrobial Peptide-Armored Composite Hydrogel Wound Dressing for Specific Removal and Efficient Eradication ofInternational journal of molecular sciences · 2023Article
- Aptamers: A prospective tool for infectious diseases diagnosis.Journal of clinical laboratory analysis · 2022Review
- Polyclonal Aptamer Libraries from a FluRoot-SELEX for the Specific Labeling of the Apical and Elongation/Differentiation Zones ofInternational journal of molecular sciences · 2022Article
- A Polyclonal Selex Aptamer Library Directly Allows Specific Labelling of the Human Gut BacteriumMolecules (Basel, Switzerland) · 2022Article
- A Polyclonal SELEX Aptamer Library Allows Differentiation ofJournal of fungi (Basel, Switzerland) · 2022Article
- A Polyclonal Aptamer Library for the Specific Binding of the Gut BacteriumInternational journal of molecular sciences · 2022Article
- Selection and Characterization of DNA Aptamers for Constructing Aptamer-AuNPs Colorimetric Method for Detection of AFM1.Foods (Basel, Switzerland) · 2022Article
- Polyclonal Aptamers for Specific Fluorescence Labeling and Quantification of the Health Relevant Human Gut BacteriumMicroorganisms · 2021Article
- BSA Hydrogel Beads Functionalized with a Specific Aptamer Library for CapturingInternational journal of molecular sciences · 2021Article
- FluCell-SELEX Aptamers as Specific Binding Molecules for Diagnostics of the Health Relevant Gut BacteriumInternational journal of molecular sciences · 2021Article
- The Diversity of a Polyclonal FluCell-SELEX Library Outperforms Individual Aptamers as Emerging Diagnostic Tools for the Identification of Carbapenem Resistant Pseudomonas aeruginosa.Chemistry (Weinheim an der Bergstrasse, Germany) · 2020Article
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
Abstract
Textbook procedures require the use of individual aptamers enriched in SELEX libraries which are subsequently chemically synthesized after their biochemical characterization. Here we show that this reduction of the available sequence space of large libraries and thus the diversity of binding molecules reduces the labelling efficiency and fidelity of selected single aptamers towards different strains of the human pathogen Pseudomonas aeruginosa compared to a polyclonal aptamer library enriched by a whole-cell-SELEX involving fluorescent aptamers. The library outperformed single aptamers in reliable and specific targeting of different clinically relevant strains, allowed to inhibit virulence associated cellular functions and identification of bound cell surface targets by aptamer based affinity purification and mass spectrometry. The stunning ease of this FluCell-SELEX and the convincing performance of the P. aeruginosa specific library may pave the way towards generally new and efficient diagnostic techniques based on polyclonal aptamer libraries not only in clinical microbiology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.