ArticleEmerging microbes & infections2020
A porcine macrophage cell line that supports high levels of replication of OURT88/3, an attenuated strain of African swine fever virus.
Article in Emerging microbes & infections, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed.
- Suidae iPSC-derived macrophages as models for investigating susceptibility and resilience to African swine fever virus.Stem cell reports · 2026Article
- Cell Culture Adaptation of Porcine Group A Rotavirus: Advances and Challenges for Vaccine Development.Viruses · 2026Review
- Evaluation of Cryopreserved Primary Swine Macrophages as a Substrate for African Swine Fever Virus Replication.Methods and protocols · 2026Article
- Hope and hurdles: unlocking the potential of modified live virus vaccines for African swine fever.Emerging microbes & infections · 2025Review
- Evolution of Porcine Virus Isolation: Guidelines for Practical Laboratory Application.Microorganisms · 2025Review
- Synergy between pluripotent stem cell-derived macrophages and self-renewing macrophages: Envisioning a promising avenue for the modelling and cell therapy of infectious diseases.Cell proliferation · 2025Review
- Propagation and Sequencing of African Swine Fever Virus on Porcine-Derived Buffy Coat Fraction Cells.Transboundary and emerging diseases · 2025Article
- The Potential of Disabled Infectious Single Cycle (DISC) Virus Platforms for Next Generation African Swine Fever Vaccine Development.Transboundary and emerging diseases · 2025Review
- Article
- Six adenoviral vectored African swine fever virus genes protect against fatal disease caused by genotype I challenge.Journal of virology · 2024Article
- African Swine Fever Diagnosis in Africa: Challenges and Opportunities.Pathogens (Basel, Switzerland) · 2024Review
- Recent progress on gene-deleted live-attenuated African swine fever virus vaccines.NPJ vaccines · 2024Review
- CA-CAS-01-A: A Permissive Cell Line for Isolation and Live Attenuated Vaccine Development Against African Swine Fever Virus.Journal of microbiology (Seoul, Korea) · 2024Article
- Advanced Strategies for Developing Vaccines and Diagnostic Tools for African Swine Fever.Viruses · 2023Review
- Review
- Attenuated African swine fever virus through serial passaging of viruses in cell culture: a brief review on the knowledge gathered during 60 years of research.Virus genes · 2023Review
- Vaccines for African swine fever: an update.Frontiers in microbiology · 2023Review
- African swine fever control and prevention: an update on vaccine development.Emerging microbes & infections · 2022Review
- Article
- Cell Lines for the Development of African Swine Fever Virus Vaccine Candidates: An Update.Vaccines · 2022Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
The main target cells for African swine fever virus (ASFV) replication in pigs are of monocyte macrophage lineage and express markers typical of the intermediate to late stages of differentiation. The lack of a porcine cell line, which accurately represents these target cells, limits research on virus host interactions and the development of live-attenuated vaccine strains. We show here that the continuously growing, growth factor dependent ZMAC-4 porcine macrophage cell line is susceptible to infection with eight different field isolates of ASFV. Replication in ZMAC-4 cells occurred with similar kinetics and to similar high titres as in primary porcine bone marrow cells. In addition we showed that twelve passages of an attenuated strain of ASFV, OURT88/3, in ZMAC-4 cells did not reduce the ability of this virus to induce protection against challenge with virulent virus. Thus, the ZMAC-4 cells provide an alternative to primary cells for ASFV replication.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.