ArticleAutophagy2021
Atg21 organizes Atg8 lipidation at the contact of the vacuole with the phagophore.
Article in Autophagy, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 38 citations in OpenAlex.
- The autophagic and non-autophagic functions of theAutophagy · 2026Article
- Formation and function of a novel Atg21-retromer complex inAutophagy · 2026Article
- Full length TECPR1 displays 'cis' Dysferlin domain architecture.bioRxiv : the preprint server for biology · 2026Article
- Phase separation promotes Atg8 lipidation and vesicle condensation for autophagy progression.Nature structural & molecular biology · 2025Article
- Molecular bases of the interactions of ATG16L1 with FIP200 and ATG8 family proteins.Nature communications · 2025Article
- When an underdog becomes a major player: the role of protein structural disorder in the Atg8 conjugation system.Autophagy · 2024Review
- Coiled-coil-mediated dimerization of Atg16 is required for binding to the PROPPIN Atg21.Open biology · 2023Article
- Topology and Function of theCells · 2023Article
- Exosome-Autophagy Crosstalk in Enveloped Virus Infection.International journal of molecular sciences · 2023Review
- Autophagic structures revealed by cryo-electron tomography: new clues about autophagosome biogenesis.Autophagy · 2023Article
- ATG16L1 adopts a dual-binding site mode to interact with WIPI2b in autophagy.Science advances · 2023Article
- Structural biology of the Atg8 and Atg12 conjugation systems.Autophagy reports · 2023Review
- Article
- Role of ATG7-dependent non-autophagic pathway in angiogenesis.Frontiers in pharmacology · 2023Review
- Membrane Contact Sites in Autophagy.Cells · 2022Review
- Vps21 Directs the PI3K-PI(3)P-Atg21-Atg16 Module to Phagophores via Vps8 for Autophagy.International journal of molecular sciences · 2022Article
- Theater in the Self-Cleaning Cell: Intrinsically Disordered Proteins or Protein Regions Acting with Membranes in Autophagy.Membranes · 2022Review
- Molecular regulation of autophagosome formation.Biochemical Society transactions · 2022Review
- Spatial control of avidity regulates initiation and progression of selective autophagy.Nature communications · 2021Article
- Article
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Coupling of Atg8 to phosphatidylethanolamine is crucial for the expansion of the crescent-shaped phagophore during cargo engulfment. Atg21, a PtdIns3P-binding beta-propeller protein, scaffolds Atg8 and its E3-like complex Atg12-Atg5-Atg16 during lipidation. The crystal structure of Atg21, in complex with the Atg16 coiled-coil domain, showed its binding at the bottom side of the Atg21 beta-propeller. Our structure allowed detailed analyses of the complex formation of Atg21 with Atg16 and uncovered the orientation of the Atg16 coiled-coil domain with respect to the membrane. We further found that Atg21 was restricted to the phagophore edge, near the vacuole, known as the vacuole isolation membrane contact site (VICS). We identified a specialized vacuolar subdomain at the VICS, typical of organellar contact sites, where the membrane protein Vph1 was excluded, while Vac8 was concentrated. Furthermore, Vac8 was required for VICS formation. Our results support a specialized organellar contact involved in controlling phagophore elongation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.