ArticleJournal of animal science2020
Vaginal bacterial community composition and concentrations of estradiol at the time of artificial insemination in Brangus heifers.
Article in Journal of animal science, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.
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Who cites it
25 citing papers in PubMed, 1 synthesis or guideline pooled it, 41 citations in OpenAlex.
- Does the Use of a Sanitary Sheath During Artificial Insemination Improve Pregnancy Outcomes in Cattle? A Systematic Review With Meta-Analysis.Reproduction in domestic animals = Zuchthygiene · 2026Pooled it
- Characterization of the vaginal bacterial microbiota of sheep exhibiting abortion or repeat breeder syndrome.Veterinary and animal science · 2026Article
- Vaginal Microbiota Composition and Its Relationship with Fertility in Repeat Breeder Dairy Cows.Biology · 2026Article
- Article
- Relationship between the composition of vaginal bacterial populations and the reproductive stage in captive collared peccaries.Reproduction & fertility · 2025Article
- Evaluating the inoculating bacterial communities within the bovine neonatal respiratory tract after applying an ablation technique to the dam vaginal microbiota prior to parturition.Journal of animal science · 2025Article
- Microbial Gatekeepers of Fertility in the Female Reproductive Microbiome of Cattle.International journal of molecular sciences · 2024Review
- Reproductive Tract Microbiota of Mares.Veterinary sciences · 2024Review
- Updates and Current Challenges in Reproductive Microbiome: A Comparative Analysis between Cows and Women.Animals : an open access journal from MDPI · 2024Review
- Dynamic principles of the microbiome and the bovine vagina: a review.Frontiers in microbiology · 2024Review
- Bovine reproductive tract and microbiome dynamics: current knowledge, challenges, and its potential to enhance fertility in dairy cows.Frontiers in microbiomes · 2024Review
- Temporal changes in ewe vaginal microbiota throughout gestation.Frontiers in microbiology · 2024Article
- Vaginal Microbiome Dynamics of Cows in Different Parities.Animals : an open access journal from MDPI · 2023Article
- Role of Date Palm Pollen on Heifer's Puberty and Maturity in Iraq.Archives of Razi Institute · 2023Article
- Reproductive Microbiomes in Domestic Livestock: Insights Utilizing 16S rRNA Gene Amplicon Community Sequencing.Animals : an open access journal from MDPI · 2023Review
- Reproductive microbiome and cytokine profiles associated with fertility outcomes of postpartum beef cows.Journal of animal science · 2023Article
- Description of the vaginal microbiota in nulliparous ewes during natural mating and pregnancy: preliminary signs of the male preputial microbiota modulation.Frontiers in microbiology · 2023Article
- Bovine neonatal microbiome origins: a review of proposed microbial community presence from conception to colostrum.Translational animal science · 2023Article
- Role of Genital Tract Bacteria in Promoting Endometrial Health in Cattle.Microorganisms · 2022Article
- Evaluation of Host Depletion and Extraction Methods for Shotgun Metagenomic Analysis of Bovine Vaginal Samples.Microbiology spectrum · 2022Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The knowledge surrounding the bovine vaginal microbiota and its implications on fertility and reproductive traits remains incomplete. The objective of the current study was to characterize the bovine vaginal bacterial community and estradiol concentrations at the time of artificial insemination (AI). Brangus heifers (n = 78) underwent a 7-d Co-Synch + controlled internal drug release estrus synchronization protocol. At AI, a double-guarded uterine culture swab was used to sample the anterior vaginal tract. Immediately after swabbing the vaginal tract, blood samples were collected by coccygeal venipuncture to determine concentrations of estradiol. Heifers were retrospectively classified as pregnant (n = 29) vs. nonpregnant (n = 49) between 41 and 57 d post-AI. Additionally, heifers were classified into low (1.1 to 2.5 pg/mL; n = 21), medium (2.6 to 6.7 pg/mL; n = 30), and high (7.2 to 17.6 pg/mL; n = 27) concentration of estradiol. The vaginal bacterial community composition was determined through sequencing of the V4 region from the 16S rRNA gene using the Illumina Miseq platform. Alpha diversity was compared via ANOVA and beta diversity was compared via PERMANOVA. There were no differences in the Shannon diversity index (alpha diversity; P = 0.336) or Bray-Curtis dissimilarity (beta diversity; P = 0.744) of pregnant vs. nonpregnant heifers. Overall, bacterial community composition in heifers with high, medium, or low concentrations of estradiol did not differ (P = 0.512). While no overall compositional differences were observed, species-level differences were present within pregnancy status and estradiol concentration groups. The implications of these species-level differences are unknown, but these differences could alter the vaginal environment thereby influencing fertility and vaginal health. Therefore, species-level changes could provide better insight rather than overall microbial composition in relation to an animal's reproductive health.
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