ArticleProceedings of the National Academy of Sciences of the United States of America2020
Molecular height measurement by cell surface optical profilometry (CSOP).
Article in Proceedings of the National Academy of Sciences of the United States of America, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 40 citations in OpenAlex.
- Flow transports extracellular lipid-anchored proteins across the surface of living COS-7 cells.Biophysical journal · 2026Article
- SurFlex microscopy: Measuring flexibility of surface-tethered biomolecules.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Flow transports extracellular lipid-anchored proteins across the surface of living COS-7 cells.bioRxiv : the preprint server for biology · 2025Article
- Cell surface crowding is a tunable energetic barrier to cell-cell fusion.Nature communications · 2025Article
- Multiscale Simulations of Membrane Adhesion Mediated by CD47-SIRPα Complexes.Journal of chemical theory and computation · 2025Article
- Leucine zipper-based SAIM imaging identifies therapeutic agents to disrupt the cancer cell glycocalyx for enhanced immunotherapy.bioRxiv : the preprint server for biology · 2024Article
- Functional Divergence in the Affinity and Stability of Non-Canonical Cysteines and Non-Canonical Disulfide Bonds: Insights from a VHH and VNAR Study.International journal of molecular sciences · 2024Article
- A new method to experimentally quantify dynamics of initial protein-protein interactions.Communications biology · 2024Article
- A framework to validate fluorescently labeled DNA-binding proteins for single-molecule experiments.Cell reports methods · 2023Article
- Review
- Engineered molecular sensors for quantifying cell surface crowding.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Antibody binding reports spatial heterogeneities in cell membrane organization.Nature communications · 2023Article
- Quantifying cytoskeletal organization from optical microscopy data.Frontiers in cell and developmental biology · 2023Review
- Measurement of Molecular Height Using Cell Surface Optical Profilometry (CSOP).Methods in molecular biology (Clifton, N.J.) · 2023Article
- Cell-Derived Vesicles with Increased Stability and On-Demand Functionality by Equipping Their Membrane with a Cross-Linkable Copolymer.Advanced healthcare materials · 2022Article
- Influence of the extracellular domain size on the dynamic behavior of membrane proteins.Biophysical journal · 2022Article
- Article
- The interplay between membrane topology and mechanical forces in regulating T cell receptor activity.Communications biology · 2022Review
- Glycocalyx Curving the Membrane: Forces Emerging from the Cell Exterior.Annual review of cell and developmental biology · 2021Review
- Cell-cell contact landscapes inProceedings of the National Academy of Sciences of the United States of America · 2021Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
The physical dimensions of proteins and glycans on cell surfaces can critically affect cell function, for example, by preventing close contact between cells and limiting receptor accessibility. However, high-resolution measurements of molecular heights on native cell membranes have been difficult to obtain. Here we present a simple and rapid method that achieves nanometer height resolution by localizing fluorophores at the tip and base of cell surface molecules and determining their separation by radially averaging across many molecules. We use this method, which we call cell surface optical profilometry (CSOP), to quantify the height of key multidomain proteins on a model cell, as well as to capture average protein and glycan heights on native cell membranes. We show that average height of a protein is significantly smaller than its contour length, due to thermally driven bending and rotation on the membrane, and that height strongly depends on local surface and solution conditions. We find that average height increases with cell surface molecular crowding but decreases with solution crowding by solutes, both of which we confirm with molecular dynamics simulations. We also use experiments and simulations to determine the height of an epitope, based on the location of an antibody, which allows CSOP to profile various proteins and glycans on a native cell surface using antibodies and lectins. This versatile method for profiling cell surfaces has the potential to advance understanding of the molecular landscape of cells and the role of the molecular landscape in cell function.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.