Evidence map›Paper›PMID 32513272›Full record

ArticleStem cell research & therapy2020

Bone marrow mesenchymal stem cell-derived endothelial cells increase capillary density and accelerate angiogenesis in mouse hindlimb ischemia model.

Ziping Yao, Huihui Liu, Min Yang, Yun Bai, Bihui Zhang, Chengen Wang, Ziguang Yan, Guochen Niu, Yinghua Zou, Yuan Li

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell research & therapy, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed, 1 pooled it
3.6field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed, 1 synthesis or guideline pooled it, 41 citations in OpenAlex.

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  20. Vascular Stem/Progenitor Cells in Vessel Injury and Repair.Frontiers in cardiovascular medicine · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 1 country.

Ziping YaoDepartment of Interventional Radiology and Vascular Surgery, Peking University First Hospital, No. 8 Xishiku Street, Beijing, 100034, China.
Huihui LiuDepartment of Hematology, Peking University First Hospital, No. 8 Xishiku Street, Beijing, 100034, China.
Min YangDepartment of Interventional Radiology and Vascular Surgery, Peking University First Hospital, No. 8 Xishiku Street, Beijing, 100034, China.
Yun BaiDepartment of Cell Biology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, China.
Bihui ZhangDepartment of Interventional Radiology and Vascular Surgery, Peking University First Hospital, No. 8 Xishiku Street, Beijing, 100034, China.
Chengen WangDepartment of Minimally Invasive Tumor Therapies Center, National Center of Gerontology, Beijing Hospital, Beijing, China.
Ziguang YanDepartment of Interventional Radiology and Vascular Surgery, Peking University First Hospital, No. 8 Xishiku Street, Beijing, 100034, China.
Guochen NiuDepartment of Interventional Radiology and Vascular Surgery, Peking University First Hospital, No. 8 Xishiku Street, Beijing, 100034, China.
Yinghua ZouDepartment of Interventional Radiology and Vascular Surgery, Peking University First Hospital, No. 8 Xishiku Street, Beijing, 100034, China. zouyinghuabdyy@sina.com.
Yuan LiDepartment of Hematology, Peking University First Hospital, No. 8 Xishiku Street, Beijing, 100034, China. drliyuan75@163.com.
Peking University · CNPeking University First Hospital · CNBeijing Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundMesenchymal stem cells (MSCs) can improve limb perfusion and increase vessel density in a murine model of hindlimb ischemia. But low engraftment rate of those cells limited their therapeutic effect. Endothelial cells (ECs) play an important role in neovascularization. And MSCs can differentiate into ECs in vitro. The aim of this study is to investigate if EC differentiation of MSCs in vitro before transplantation is effective in improving therapeutic outcomes in the treatment of ischemic disease in a murine ischemia animal model.

methodsMSCs were isolated from the bone marrow of EGFP-transgenic mice by density gradient centrifugation. The identity of the MSCs was determined by their cluster of differentiation (CD) marker profile by flow cytometry. Inducing medium containing a few cytokines was applied to induce the MSCs to differentiate into ECs. Endothelial differentiation was quantitatively evaluated using flow cytometry, quantitative real-time PCR (qRT-PCR), immunofluorescence, Matrigel tube formation assay, and Dil-labeled acetylated low-density lipoprotein uptake assay. Mouse hindlimb ischemia model was made by excision of the femoral artery. Uninduced EGFP+ MSCs, induced EGFP+ MSCs, and PBS were intramuscularly injected into the gastrocnemius following ischemia no later than 24 h after operation. Restoration of blood flow and muscle function was evaluated by laser Doppler perfusion imaging. Immunofluorescence was conducted to evaluate the engraftment of transplanted MSCs. Histological analysis was performed to evaluate blood vessel formation.

resultsInduced EGFP+ MSCs expressed endothelial markers and exhibited tube formation capacity. Mice in the induced EGFP+ MSCs group had a better blood perfusion recovery, enhanced vessel densities, higher engraftment, and improved function of the ischemic limb than those in the uninduced EGFP+ MSCs or PBS groups.

conclusionsThis study reveals that after short-term pre-treatment in the EC-inducing medium, induced MSCs acquire stronger vessel formation capability and enhanced angiogenic therapeutic effect in the murine hindlimb ischemia model.

Indexed as

Mesenchymal Stem CellsMesenchymal Stem Cell TransplantationAnimalsDisease Models, AnimalEndothelial CellsHindlimbIschemiaMiceMice, TransgenicNeovascularization, PhysiologicAngiogenesisEndothelial cellsEngraftmentHindlimb ischemia modelMesenchymal stem cells

Identifiers

PMID32513272
PMCPMC7278145
OpenAlexW3034030369

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.