Evidence map›Paper›PMID 32513270›Full record

ArticleStem cell research & therapy2020

Hypoxia-challenged MSC-derived exosomes deliver miR-210 to attenuate post-infarction cardiac apoptosis.

Hao Cheng, Shufu Chang, Rende Xu, Lu Chen, Xiaoyue Song, Jian Wu, Juying Qian, Yunzeng Zou, Jianying Ma

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell research & therapy, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 108 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
108citing papers in PubMed, 3 pooled it
9.4field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

108 citing papers in PubMed, 3 syntheses or guidelines pooled it, 180 citations in OpenAlex.

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  4. Unifying and unique roles of non-coding RNA biomarkers in liver and heart fibrosis.Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie · 2026
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48 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Hao ChengDepartment of Cardiology, Zhongshan Hospital, Fudan University, 1609 Xietu Road, Shanghai, 200032, China.ORCID 0000-0003-1360-6290
Shufu ChangDepartment of Cardiology, Zhongshan Hospital, Fudan University, 1609 Xietu Road, Shanghai, 200032, China.
Rende XuDepartment of Cardiology, Zhongshan Hospital, Fudan University, 1609 Xietu Road, Shanghai, 200032, China.
Lu ChenDepartment of Cardiology, Zhongshan Hospital, Fudan University, 1609 Xietu Road, Shanghai, 200032, China.
Xiaoyue SongDepartment of Cardiology, Zhongshan Hospital, Fudan University, 1609 Xietu Road, Shanghai, 200032, China.
Jian WuShanghai Institute of Cardiovascular Diseases, Zhongshan Hospital and Institute of Biomedical Sciences, Fudan University, 180 Feng Lin Road, Shanghai, 20032, China.
Juying QianDepartment of Cardiology, Zhongshan Hospital, Fudan University, 1609 Xietu Road, Shanghai, 200032, China.
Yunzeng ZouShanghai Institute of Cardiovascular Diseases, Zhongshan Hospital and Institute of Biomedical Sciences, Fudan University, 180 Feng Lin Road, Shanghai, 20032, China.
Jianying MaDepartment of Cardiology, Zhongshan Hospital, Fudan University, 1609 Xietu Road, Shanghai, 200032, China. ma.jianying@zs-hospital.sh.cn.
Fudan University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundMyocardial infarction (MI) is a major cause of death worldwide. Although percutaneous coronary intervention and coronary artery bypass grafting can prolong life, cardiac damage persists. In particular, cardiomyocytes have no regenerative capacity. Mesenchymal stem cells (MSCs) are attractive candidates for the treatment of MI. The manner by which MSCs exert a beneficial effect upon injured cells is a source of continued study.

methodsAfter the isolation and identification of exosomes from MSCs, the expression of miR-210 was determined by microarray chip. Subsequently, gain- and loss-function approaches were conducted to detect the role of exosomes and exosomal-miR-210 in cell proliferation and apoptosis of cardiomyocytes, as well as the MI in vivo. Dual-Luciferase Report Gene System was used to demonstrate the target gene of miR-210.

resultsWe tested the hypothesis that MSC-derived exosomes transfer specific miRNA to protect cardiomyocytes from apoptotic cell death. Interestingly, direct cardiac injection of MSC exosomes reduced infarct size and improved heart function after coronary ligation. In vitro, the MSC exosomes enhanced cardiomyocyte survival to hypoxia. Confirmation of exosome uptake in myocytes was confirmed. Dual-luciferase reporter assay implicated miR-210 as a mediator of the therapeutic effect and AIFM3 as a downstream target. Treatment with miR-210 overexpressing MSC exosomes improved myocyte protection to both in vitro and in vivo stress. Furthermore, the endogenous and exogenous miR-210 had the same therapeutic effects.

conclusionThese results demonstrated that the beneficial effects offered by MSC-exosomes transplantation after MI are at least partially because of excreted exosome containing mainly miR-210.

Indexed as

ExosomesMicroRNAsMyocardial InfarctionApoptosisHumansHypoxiaMyocytes, CardiacMicroRNAsMIRN210 microRNA, humanApoptosisExosomeHypoxiaMesenchymal stem cellsMyocardial infarction

Identifiers

PMID32513270
PMCPMC7278138
OpenAlexW3034011377

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.