Evidence map›Paper›PMID 32512915›Full record

ArticleMicromachines2020

Cell Culture on Low-Fluorescence and High-Resolution Photoresist.

Hidetaka Ueno, Katsuya Maruo, Masatoshi Inoue, Hidetoshi Kotera, Takaaki Suzuki

Open access · goldAbstract read
In one paragraph

Article in Micromachines, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.7field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 4 institutions in 1 country.

Hidetaka UenoDivision of Mechanical Science and Technology, Gunma University, Kiryu 376-8515, Japan.
Katsuya MaruoInnovation Park, Daicel Corporation, Himeji 671-1283, Japan.
Masatoshi InoueDivision of Intelligent Mechanical Systems Engineering, Kagawa University, Takamatsu 761-0396, Japan.
Hidetoshi KoteraRIKEN, Wako 351-0198, Japan.
Takaaki SuzukiDivision of Mechanical Science and Technology, Gunma University, Kiryu 376-8515, Japan.ORCID 0000-0003-2574-7971
Gunma University · JPDaicel (Japan) · JPKagawa University · JPNippon Soken (Japan) · JP

Funding

Japan Science and Technology Agency JPMJPR15R3, JPMJCR19Q2Japan Society for the Promotion of Science 16J12039, JP17H03196
6 · The paper itself

Abstract

2D and 3D topographic cues made of photoresist, a polymer, are used for cell culture and cell analysis. Photoresists used for cell analysis provide the surface conditions necessary for proper cell growth, along with patterning properties of a wide range and high precision, and low auto-fluorescence that does not affect fluorescence imaging. In this study, we developed a thick negative photoresist SJI-001 possessing the aforementioned properties. We evaluated the surface conditions of SJI-001 affecting cell culture. First, we studied the wettability of SJI-001, which was changed by plasma treatment, conducted as a pretreatment on a plastic substrate before cell seeding. SJI-001 was more chemically stable than SU-8 used for fabricating the micro-electromechanical systems (MEMS). Furthermore, the doubling time and adhesion rate of adherent HeLa cells cultured on untreated SJI-001 were 25.2 h and 74%, respectively, thus indicating its suitability for cell culture over SU-8. In addition, we fabricated a cell culture plate with a 3D lattice structure, three micrometers in size, using SJI-001. HeLa cells seeded on this plate remained attached over five days. Therefore, SJI-001 exhibits surface conditions suitable for cell culture and has several bioapplications including microstructures and cell chips for cell culture and cell analysis.

Indexed as

cell analysiscell chiphigh resolutionlow-fluorescencemicro and nanotechnologyphotoresist

Identifiers

PMID32512915
PMCPMC7345055
OpenAlexW3033538431

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.