Evidence map›Paper›PMID 32511516›Full record

ArticlemedRxiv : the preprint server for health sciences2020

Robust ACE2 protein expression localizes to the motile cilia of the respiratory tract epithelia and is not increased by ACE inhibitors or angiotensin receptor blockers.

Ivan T Lee, Tsuguhisa Nakayama, Chien-Ting Wu, Yury Goltsev, Sizun Jiang, Phillip A Gall, Chun-Kang Liao, Liang-Chun Shih, Christian M Schürch, David R McIlwain and 26 more

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in medRxiv : the preprint server for health sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 37 citations in OpenAlex.

  1. Article
  2. First contact: the role of respiratory cilia in host-pathogen interactions in the airways.American journal of physiology. Lung cellular and molecular physiology · 2020
    Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

36 authors at 9 institutions in 4 countries.

Ivan T LeeDepartment of Pathology, Stanford University School of Medicine, Stanford, California 94305, USA.
Tsuguhisa NakayamaDepartment of Otolaryngology-Head and Neck Surgery, Stanford University School of Medicine, 801 Welch Road, Stanford, CA, USA.
Chien-Ting WuBaxter Laboratory, Department of Microbiology & Immunology, Stanford University School of Medicine, Stanford, California, USA.
Yury GoltsevDepartment of Pathology, Stanford University School of Medicine, Stanford, California 94305, USA.
Sizun JiangDepartment of Pathology, Stanford University School of Medicine, Stanford, California 94305, USA.
Phillip A GallDepartment of Otolaryngology-Head and Neck Surgery, Stanford University School of Medicine, 801 Welch Road, Stanford, CA, USA.
Chun-Kang LiaoDepartment of Otolaryngology, National Taiwan University Hospital, Taipei, Taiwan.
Liang-Chun ShihDepartment of Otorhinolaryngology, China Medical University Hospital, Taichung, Taiwan.
Christian M SchürchDepartment of Pathology, Stanford University School of Medicine, Stanford, California 94305, USA.
David R McIlwainDepartment of Pathology, Stanford University School of Medicine, Stanford, California 94305, USA.
Pauline ChuDepartment of Pathology, Stanford University School of Medicine, Stanford, California 94305, USA.
Nicole A BorchardDepartment of Otolaryngology-Head and Neck Surgery, Stanford University School of Medicine, 801 Welch Road, Stanford, CA, USA.
David ZarabandaDepartment of Otolaryngology-Head and Neck Surgery, Stanford University School of Medicine, 801 Welch Road, Stanford, CA, USA.
Sachi S DholakiaDepartment of Otolaryngology-Head and Neck Surgery, Stanford University School of Medicine, 801 Welch Road, Stanford, CA, USA.
Angela YangDepartment of Otolaryngology-Head and Neck Surgery, Stanford University School of Medicine, 801 Welch Road, Stanford, CA, USA.
Dayoung KimDepartment of Otolaryngology-Head and Neck Surgery, Stanford University School of Medicine, 801 Welch Road, Stanford, CA, USA.
Tomoharu KanieBaxter Laboratory, Department of Microbiology & Immunology, Stanford University School of Medicine, Stanford, California, USA.
Chia-Der LinDepartment of Otorhinolaryngology, China Medical University Hospital, Taichung, Taiwan.
Ming-Hsui TsaiDepartment of Otorhinolaryngology, China Medical University Hospital, Taichung, Taiwan.
Katie M PhillipsDepartment of Otolaryngology-Head and Neck Surgery, Stanford University School of Medicine, 801 Welch Road, Stanford, CA, USA.
Raymond KimDepartment of Otolaryngology-Head and Neck Surgery, Stanford University School of Medicine, 801 Welch Road, Stanford, CA, USA.
Jonathan B OverdevestDepartment of Otolaryngology-Head and Neck Surgery, Stanford University School of Medicine, 801 Welch Road, Stanford, CA, USA.
Matthew A TylerDepartment of Otolaryngology-Head and Neck Surgery, Stanford University School of Medicine, 801 Welch Road, Stanford, CA, USA.
Carol H YanDepartment of Otolaryngology-Head and Neck Surgery, Stanford University School of Medicine, 801 Welch Road, Stanford, CA, USA.
Chih-Feng LinDepartment of Otolaryngology, National Taiwan University Hospital, Taipei, Taiwan.
Yi-Tsen LinDepartment of Otolaryngology, National Taiwan University Hospital, Taipei, Taiwan.
Da-Tian BauGraduate Institute of Biomedical Sciences, China Medical University, Taichung, Taiwan.
Gregory J TsaySchool of Medicine, China Medical University, Taichung, Taiwan.
Zara M PatelDepartment of Otolaryngology-Head and Neck Surgery, Stanford University School of Medicine, 801 Welch Road, Stanford, CA, USA.
Yung-An TsouDepartment of Otorhinolaryngology, China Medical University Hospital, Taichung, Taiwan.
Chih-Jaan TaiDepartment of Otorhinolaryngology, China Medical University Hospital, Taichung, Taiwan.
Te-Huei YehDepartment of Otolaryngology, National Taiwan University Hospital, Taipei, Taiwan.
Peter H HwangDepartment of Otolaryngology-Head and Neck Surgery, Stanford University School of Medicine, 801 Welch Road, Stanford, CA, USA.
Garry P NolanDepartment of Pathology, Stanford University School of Medicine, Stanford, California 94305, USA.
Jayakar V NayakDepartment of Otolaryngology-Head and Neck Surgery, Stanford University School of Medicine, 801 Welch Road, Stanford, CA, USA.
Peter K JacksonBaxter Laboratory, Department of Microbiology & Immunology, Stanford University School of Medicine, Stanford, California, USA.
Stanford Medicine · USChina Medical University · TWStanford University · USTÜBİTAK National Observatory · TRJoint Graduate School of Energy and Environment · THNational Taiwan University Hospital · TWCity University of New York · USUniversity of Minnesota · USUniversity of San Diego · US

Funding

Using a tonsil organoid system to probe conditions for the induction of protective antibody and T cell responses to influenza.U19AI057229 · NIAID · STANFORD UNIVERSITY · PI Mark Morris Davis · 2003 to 2026
$88.5M
Stanford Islet Research CoreP30DK116074 · NIDDK · STANFORD UNIVERSITY · PI Seung K Kim · 2017 to 2026
$19.5M
Defining Mechanisms of Viral Persistence in Situ at the Single-Cell LevelR01AI149672 · NIAID · OREGON HEALTH & SCIENCE UNIVERSITY · PI ESTES, JACOB D · 2020 to 2024
$4.0M
NIAID NIH HHS R01 AI149672NIAID NIH HHS U19 AI057229NIDDK NIH HHS P30 DK116074
6 · The paper itself

Abstract

We investigated the expression and subcellular localization of the SARS-CoV-2 receptor, angiotensin-converting enzyme 2 (ACE2), within the upper (nasal) and lower (pulmonary) respiratory tracts of healthy human donors. We detected ACE2 protein expression within the cilia organelle of ciliated airway epithelial cells, which likely represents the initial or early subcellular site of SARS-CoV-2 viral entry during respiratory transmission. We further determined whether ACE2 expression in the cilia of upper respiratory cells was influenced by patient demographics, clinical characteristics, co-morbidities, or medication use, and found no evidence that the use of angiotensin-converting enzyme inhibitors (ACEI) or angiotensin II receptor blockers (ARBs) increases ACE2 protein expression.

Identifiers

PMID32511516
PMCPMC7273284
OpenAlexW3024455343

What OpenQuestion holds

Textmetadata
LicenceCC BY-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.