Evidence map›Paper›PMID 32511318›Full record

ArticlebioRxiv : the preprint server for biology2020

Topoisomerase III-ß is required for efficient replication of positive-sense RNA viruses.

K Reddisiva Prasanth, Minato Hirano, W Samuel Fagg, Eileen T McAnarney, Chao Shan, Xuping Xie, Adam Hage, Colette A Pietzsch, Alexander Bukreyev, Ricardo Rajsbaum and 5 more

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 5 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

15 authors at 3 institutions in 2 countries.

K Reddisiva PrasanthDepartment of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, Texas, USA.
Minato HiranoDepartment of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, Texas, USA.
W Samuel FaggDepartment of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, Texas, USA.
Eileen T McAnarneyDepartment of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas, USA.
Chao ShanDepartment of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, Texas, USA.
Xuping XieDepartment of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, Texas, USA.
Adam HageDepartment of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas, USA.
Colette A PietzschDepartment of Pathology, University of Texas Medical Branch, Galveston, Texas, USA.
Alexander BukreyevDepartment of Pathology, University of Texas Medical Branch, Galveston, Texas, USA.
Ricardo RajsbaumDepartment of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas, USA.
Pei-Yong ShiDepartment of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, Texas, USA.
Mark T BedfordDepartment of Epigenetics and Molecular Carcinogenesis, MD Anderson Cancer Center, Smithville, Texas, USA.
Shelton S BradrickDepartment of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, Texas, USA.
Vineet MenacheryDepartment of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas, USA.
Mariano A Garcia-BlancoDepartment of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, Texas, USA.
The University of Texas Medical Branch at Galveston · USDuke-NUS Medical School · SGThe University of Texas MD Anderson Cancer Center · US

Funding

Project 4 - Influenza - UABU19AI142759 · NIAID · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI WHITLEY, RICHARD J. · 2019 to 2023
$37.5M
Regulation of Ebola virus replication by the host ubiquitin systemR01AI134907 · NIAID · UNIVERSITY OF TEXAS MED BR GALVESTON · PI GIRALDO, MARIA ISABEL · 2018 to 2022
$3.1M
Biodefense Training ProgramT32AI060549 · NIAID · UNIVERSITY OF TEXAS MEDICAL BR GALVESTON · PI ASHOK K CHOPRA, Janice J Endsley · 2004 to 2026
$3.0M
Consequences and mechanism of aberrant splicing in African American prostate cancer disparitiesR01CA204806 · NCI · GEORGE WASHINGTON UNIVERSITY · PI GARCIA-BLANCO, MARIANO A., LEE, NORMAN H · 2017 to 2021
$1.8M
Competitive macrophage microRNA-RNA binding protein interactions in wound repairR01GM126412 · NIGMS · YALE UNIVERSITY · PI BENDER, JEFFREY R. · 2017 to 2020
$1.3M
NCI NIH HHS R01 CA204806NIAID NIH HHS R01 AI134907NIAID NIH HHS T32 AI060549NIAID NIH HHS U19 AI142759NIGMS NIH HHS R01 GM126412
6 · The paper itself

Abstract

Based on genome-scale loss-of-function screens we discovered that Topoisomerase III-ß (TOP3B), a human topoisomerase that acts on DNA and RNA, is required for yellow fever virus and dengue virus-2 replication. Remarkably, we found that TOP3B is required for efficient replication of all positive-sense-single stranded RNA viruses tested, including SARS-CoV-2. While there are no drugs that specifically inhibit this topoisomerase, we posit that TOP3B is an attractive anti-viral target.

Identifiers

PMID32511318
PMCPMC7239047
OpenAlexW3013061579

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.