Evidence map›Paper›PMID 32511255›Full record

ArticlePloS one2020

Azithromycin decreases Chlamydia pneumoniae-mediated Interleukin-4 responses but not Immunoglobulin E responses.

Tamar A Smith-Norowitz, Yvonne Huang, Jeffrey Loeffler, Elliot Klein, Yitzchok M Norowitz, Margaret R Hammerschlag, Rauno Joks, Stephan Kohlhoff

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 44% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Review
  2. Insights Into Host Cell Cytokines inFrontiers in immunology · 2021
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Tamar A Smith-NorowitzDepartment of Pediatrics, Division of Infectious Diseases, State University of New York Health Sciences University, Brooklyn, New York, United States of America.ORCID 0000-0002-8204-6728
Yvonne HuangDepartment of Pediatrics, Division of Infectious Diseases, State University of New York Health Sciences University, Brooklyn, New York, United States of America.
Jeffrey LoefflerDepartment of Pediatrics, Division of Infectious Diseases, State University of New York Health Sciences University, Brooklyn, New York, United States of America.
Elliot KleinDepartment of Pediatrics, Division of Infectious Diseases, State University of New York Health Sciences University, Brooklyn, New York, United States of America.
Yitzchok M NorowitzDepartment of Pediatrics, Division of Infectious Diseases, State University of New York Health Sciences University, Brooklyn, New York, United States of America.
Margaret R HammerschlagDepartment of Pediatrics, Division of Infectious Diseases, State University of New York Health Sciences University, Brooklyn, New York, United States of America.
Rauno JoksDepartment of Medicine, State University of New York Downstate Health Sciences University, Brooklyn, New York, United States of America.
Stephan KohlhoffDepartment of Pediatrics, Division of Infectious Diseases, State University of New York Health Sciences University, Brooklyn, New York, United States of America.
State University of New York · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundChlamydia pneumoniae is an obligate intracellular bacterium that causes respiratory infection. There may exist an association between C. pneumoniae, asthma, and production of immunoglobulin (Ig) E responses in vitro. Interleukin (IL-4) is required for IgE production.

objectiveWe previously demonstrated that doxycycline suppresses C. pneumoniae-induced production of IgE and IL-4 responses in peripheral blood mononuclear cells (PBMC) from asthmatic subjects. Whereas macrolides have anti-chlamydial activity, their effect on in vitro anti-inflammatory (IgE) and IL-4 responses to C. pneumoniae have not been studied.

methodsPBMC from IgE- adult atopic subjects (N = 5) were infected +/- C. pneumoniae BAL69, +/- azithromycin (0.1, 1.0 ug/mL) for 10 days. IL-4 and IgE levels were determined in supernatants by ELISA. IL-4 and IgE were detected in supernatants of PBMC (day 10).

resultsWhen azithromycin (0.1, 1.0 ug/ml) was added, IL-4 levels decreased. At low dose, IgE levels increased and at high dose, IgE levels decreased. When PBMC were infected with C. pneumoniae, both IL-4 and IgE levels decreased. Addition of azithromycin (0.1, 1.0 ug/mL) decreased IL-4 levels and had no effect on IgE levels.

conclusionsThese findings indicate that azithromycin decreases IL-4 responses but has a bimodal effect on IgE responses in PBMC from atopic patients in vitro.

Indexed as

AgedAnti-Bacterial AgentsAsthmaAzithromycinChlamydophila InfectionsChlamydophila pneumoniaeFemaleHumansHypersensitivity, ImmediateImmunoglobulin EInterleukin-4In Vitro TechniquesLeukocytes, MononuclearMaleMiddle AgedRespiratory Tract InfectionsAnti-Bacterial AgentsAzithromycinIL4 protein, humanImmunoglobulin EInterleukin-4

Identifiers

PMID32511255
PMCPMC7279567
OpenAlexW3034084858

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.