Evidence map›Paper›PMID 32510469›Full record

ArticleActa crystallographica. Section F, Structural biology communications2020

Enhanced X-ray diffraction of in vivo-grown μNS crystals by viscous jets at XFELs.

Nirupa Nagaratnam, Yanyang Tang, Sabine Botha, Justin Saul, Chufeng Li, Hao Hu, Sahba Zaare, Mark Hunter, David Lowry, Uwe Weierstall and 6 more

Open access · hybridAbstract read
In one paragraph

Article in Acta crystallographica. Section F, Structural biology communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.6field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 13 citations in OpenAlex.

  1. Article
  2. Article
  3. High-throughput structure determination of an intrinsically disordered protein using cell-free protein crystallization.Proceedings of the National Academy of Sciences of the United States of America · 2024
    Article
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors at 4 institutions in 3 countries.

Nirupa NagaratnamSchool of Molecular Sciences, Arizona State University, Tempe, AZ 85287, USA.
Yanyang TangSchool of Molecular Sciences, Arizona State University, Tempe, AZ 85287, USA.
Sabine BothaDepartment of Physics, Arizona State University, Tempe, AZ 85287, USA.
Justin SaulVirginia G. Piper Center for Personalized Diagnostics, The Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.
Chufeng LiDepartment of Physics, Arizona State University, Tempe, AZ 85287, USA.ORCID 0000-0002-2997-0903
Hao HuDepartment of Physics, Arizona State University, Tempe, AZ 85287, USA.
Sahba ZaareDepartment of Physics, Arizona State University, Tempe, AZ 85287, USA.
Mark HunterLinac Coherent Light Source, Stanford Linear Accelerator Center (SLAC) National Accelerator Laboratory, Menlo Park, CA 94025, USA.
David LowryEyring Materials Center, Arizona State University, Tempe, AZ 85287, USA.
Uwe WeierstallBiodesign Center for Applied Structural Discovery, The Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.
Nadia ZatsepinBiodesign Center for Applied Structural Discovery, The Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.ORCID 0000-0003-1757-0205
John C H SpenceBiodesign Center for Applied Structural Discovery, The Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.
Ji QiuVirginia G. Piper Center for Personalized Diagnostics, The Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.
Joshua LaBaerSchool of Molecular Sciences, Arizona State University, Tempe, AZ 85287, USA.
Petra FrommeSchool of Molecular Sciences, Arizona State University, Tempe, AZ 85287, USA.
Jose M Martin-GarciaBiodesign Center for Applied Structural Discovery, The Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.
Arizona State University · USFermi National Accelerator Laboratory · USLa Trobe University · AUUniversity of Essex · GB

Funding

Flinn Foundation 1991National Science Foundation, BioXFEL Science and Technology Center 1231306
6 · The paper itself

Abstract

μNS is a 70 kDa major nonstructural protein of avian reoviruses, which cause significant economic losses in the poultry industry. They replicate inside viral factories in host cells, and the μNS protein has been suggested to be the minimal viral factor required for factory formation. Thus, determining the structure of μNS is of great importance for understanding its role in viral infection. In the study presented here, a fragment consisting of residues 448-605 of μNS was expressed as an EGFP fusion protein in Sf9 insect cells. EGFP-μNS

Indexed as

ElectronsLasersAnimalsCrystallizationGreen Fluorescent ProteinsModels, MolecularProtein ConformationRecombinant Fusion ProteinsReoviridaeSf9 CellsViral Nonstructural ProteinsViscosityX-Ray DiffractionX-Raysenhanced green fluorescent proteinGreen Fluorescent ProteinsRecombinant Fusion ProteinsViral Nonstructural Proteinsavian reovirushigh-viscosity jetsin vivo crystallizationserial crystallographyX-ray free-electron lasersμNS

Identifiers

PMID32510469
PMCPMC7278499
OpenAlexW3032245436

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.