ArticleFrontiers in cell and developmental biology2020
A Testis-Derived Hydrogel as an Efficient Feeder-Free Culture Platform to Promote Mouse Spermatogonial Stem Cell Proliferation and Differentiation.
Article in Frontiers in cell and developmental biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 35 citations in OpenAlex.
- Modular 3D culture platform combining methylcellulose, testicular ECM, and Sertoli cells forIranian journal of basic medical sciences · 2026Article
- Microfluidic systems in testicular in vitro culture: a powerful model tool for spermatogenesis and reprotoxicity studies.Asian journal of andrology · 2025Review
- Effect of Extracellular Matrix Derived from Porcine Tissue on Stemness of Porcine Spermatogonial Stem Cells.International journal of molecular sciences · 2025Article
- Testicular extracellular matrix/gelatin-based scaffold using gas foaming to support spermatogonial stem cells.Iranian journal of basic medical sciences · 2025Article
- Hydrogel technologies in andrology: advances in research and prospective applications.Frontiers in pharmacology · 2025Review
- Engineering a 3D platform for testis bioengineering: generation and proteomic profiling of decellularized fish testicular scaffolds.Frontiers in bioengineering and biotechnology · 2025Article
- Microenvironment of spermatogonial stem cells: a key factor in the regulation of spermatogenesis.Stem cell research & therapy · 2024Review
- In vivo and in vitro sperm production: an overview of the challenges and advances in male fertility restoration.Clinical and experimental reproductive medicine · 2024Article
- H3K9me3 Levels Affect the Proliferation of Bovine Spermatogonial Stem Cells.International journal of molecular sciences · 2024Article
- The effect of epididymosomes on the development of frozen-thawed mouse spermatogonial stem cells after culture in a decellularized testicular scaffold and transplantation into azoospermic mice.Journal of assisted reproduction and genetics · 2024Article
- Mechanical properties of native and decellularized reproductive tissues: insights for tissue engineering strategies.Scientific reports · 2024Article
- Hyaluronic acid/silicon nanoparticle scaffold induces proliferation and differentiation of mouse spermatogonial stem cells transplanted to epididymal adipose tissue.Cell and tissue banking · 2024Article
- In vitro spermatogenesis in artificial testis: current knowledge and clinical implications for male infertility.Cell and tissue research · 2023Review
- The Decellularized Calf Testis: Introducing Suitable Scaffolds for Spermatogenesis Studies.International journal of fertility & sterility · 2023Article
- Advances of three-dimensional (3D) culture systems for in vitro spermatogenesis.Stem cell research & therapy · 2023Review
- 3D-printed placental-derived bioinks for skin tissue regeneration with improved angiogenesis and wound healing properties.Materials today. Bio · 2023Article
- Biomaterials for Testicular Bioengineering: How far have we come and where do we have to go?Frontiers in endocrinology · 2023Review
- A Role for Exchange of Extracellular Vesicles in Porcine Spermatogonial Co-Culture.International journal of molecular sciences · 2022Article
- Progress in germline stem cell transplantation in mammals and the potential usage.Reproductive biology and endocrinology : RB&E · 2022Review
- Future Challenges and Opportunities of Extracellular Matrix Hydrogels in Female Reproductive Medicine.International journal of molecular sciences · 2022Review
Corrections and comments
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Authors and funding
13 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fertility preservation and assisted reproductive medicine require effective culture systems for the successful proliferation and differentiation of spermatogonial stem cells (SSCs). Many SSC culture systems require the addition of feeder cells at each subculture, which is tedious and inefficient. Here, we prepared decellularized testicular matrix (DTM) from testicular tissue, which preserved essential structural proteins of testis. The DTM was then solubilized and induced to form a porous hydrogel scaffold with randomly oriented fibrillar structures that exhibited good cytocompatibility. The viability of SSCs inoculated onto DTM hydrogel scaffolds was significantly higher than those inoculated on Matrigel or laminin, and intracellular gene expression and DNA imprinting patterns were similar to that of native SSCs. Additionally, DTM promoted SSC differentiation into round spermatids. More importantly, the DTM hydrogel supported SSC proliferation and differentiation without requiring additional somatic cells. The DTM hydrogel scaffold culture system provided an alternative and simple method for culturing SSCs that eliminates potential variability and contamination caused by feeder cells. It might be a valuable tool for reproductive medicine.
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