Evidence map›Paper›PMID 32509739›Full record

ArticleFrontiers in bioengineering and biotechnology2020

Endothelial Cell Morphology Regulates Inflammatory Cells Through MicroRNA Transferred by Extracellular Vesicles.

Jiaqi Liang, Shuangying Gu, Xiuli Mao, Yiling Tan, Huanli Wang, Song Li, Yue Zhou

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in bioengineering and biotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Review
  8. Article
  9. Review
  10. Article
  11. Review
  12. Article
  13. Review
  14. Article
  15. Review
  16. Article
  17. Review
  18. Review
  19. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Jiaqi LiangShanghai Jiao Tong University Affiliated Sixth People's Hospital, School of Biomedical Engineering, Med-X Research Institution, Shanghai Jiao Tong University, Shanghai, China.
Shuangying GuShanghai Jiao Tong University Affiliated Sixth People's Hospital, School of Biomedical Engineering, Med-X Research Institution, Shanghai Jiao Tong University, Shanghai, China.
Xiuli MaoShanghai Jiao Tong University Affiliated Sixth People's Hospital, School of Biomedical Engineering, Med-X Research Institution, Shanghai Jiao Tong University, Shanghai, China.
Yiling TanShanghai Jiao Tong University Affiliated Sixth People's Hospital, School of Biomedical Engineering, Med-X Research Institution, Shanghai Jiao Tong University, Shanghai, China.
Huanli WangShanghai Jiao Tong University Affiliated Sixth People's Hospital, School of Biomedical Engineering, Med-X Research Institution, Shanghai Jiao Tong University, Shanghai, China.
Song LiDepartment of Bioengineering, Department of Medicine, University of California, Los Angeles, Los Angeles, CA, United States.
Yue ZhouShanghai Jiao Tong University Affiliated Sixth People's Hospital, School of Biomedical Engineering, Med-X Research Institution, Shanghai Jiao Tong University, Shanghai, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Vascular inflammation plays an important role in the pathogenesis and the development of cardiovascular diseases such as arteriosclerosis and restenosis, and the dysfunction of endothelial cells (ECs) may result in the activation of monocytes and other inflammatory cells. ECs exhibit an elongated morphology in the straight part of arteries but a cobblestone shape near the pro-atherogenic region such as branch bifurcation. Although the effects of hemodynamic forces on ECs have been widely studied, it is not clear whether the EC morphology affects its own function and thus the inflammatory response of monocytes. Here we showed that elongated ECs cultured on poly-(dimethyl siloxane) membrane surface with microgrooves significantly suppressed the activation of the monocytes in co-culture, in comparison to ECs with a cobblestone shape. The transfer of EC-conditioned medium to monocytes had the same effect, suggesting that soluble factors were involved in EC-monocyte communication. Further investigation demonstrated that elongated ECs upregulated the expression of anti-inflammatory microRNAs, especially miR-10a. Moreover, miR-10a was found in the extracellular vesicles (EVs) released by ECs and transferred to monocytes, and the inhibition of EV secretion from ECs repressed the upregulation of miR-10a. Consistently, the inhibition of miR-10a expression in ECs reduced their anti-inflammatory effect on monocytes. These results reveal that the EC morphology can regulate inflammatory response through EVs, which provides a basis for the design and the optimization of biomaterials for vascular tissue engineering.

Indexed as

extracellular vesiclesmicrotopologymiR-10amonocytesvascular inflammation

Identifiers

PMID32509739
PMCPMC7248333

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.