Evidence map›Paper›PMID 32509436›Full record

ArticleTranslational vision science & technology2020

Visualization of Mouse Choroidal and Retinal Vasculature Using Fluorescent Tomato Lectin Perfusion.

Chunhua Jiao, Kelsey Adler, Xiuying Liu, Weize Sun, Robert F Mullins, Elliott H Sohn

Open access · goldAbstract read
In one paragraph

Article in Translational vision science & technology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
1.6field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 23 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Not All Lectins Are Equally Suitable for Labeling Rodent Vasculature.International journal of molecular sciences · 2021
    Article
  11. Article
  12. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Chunhua JiaoDepartment of Ophthalmology and Visual Sciences, University of Iowa Hospitals & Clinics, Iowa City, IA, USA.
Kelsey AdlerCarver College of Medicine, University of Iowa, Iowa City, IA, USA.
Xiuying LiuDepartment of Ophthalmology and Visual Sciences, University of Iowa Hospitals & Clinics, Iowa City, IA, USA.
Weize SunDepartment of Ophthalmology and Visual Sciences, University of Iowa Hospitals & Clinics, Iowa City, IA, USA.
Robert F MullinsDepartment of Ophthalmology and Visual Sciences, University of Iowa Hospitals & Clinics, Iowa City, IA, USA.
Elliott H SohnDepartment of Ophthalmology and Visual Sciences, University of Iowa Hospitals & Clinics, Iowa City, IA, USA.
University of Iowa Hospitals and Clinics · USUniversity of Iowa · US

Funding

Stem Cell and Organoid CoreP30EY025580 · NEI · UNIVERSITY OF IOWA · PI Budd A Tucker · 2016 to 2026
$6.9M
Choroidal Disease Mechanisms in AMDR01EY026547 · NEI · UNIVERSITY OF IOWA · PI SOHN, ELLIOTT H · 2016 to 2020
$1.9M
NEI NIH HHS P30 EY025580NEI NIH HHS R01 EY026547
6 · The paper itself

Abstract

Purpose: To develop a reliable and simplified method to assess choroid and retinal vasculature on whole mount and cross sections in mice using tomato lectin (TL; Methods: Albino mice ( Results: Intravascular injection of TL led to consistent, robust labeling of retinal and choroidal vascular walls. On cross-sections, choriocapillaris was co-labeled with CA4 and TL. On flat mount, TL perfusion resulted in better labeling of choroidal vessels using tail/jugular vein injection compared with cardiac perfusion ( Conclusions: TL injection intravascularly can reliably label normal and ablated choroid and retinal vasculature in mouse in a quick, simple manner. Translational Relevance: These data will help to facilitate modeling in rodents for diseases such as age-related macular degeneration, diabetes, and other ischemic/angiogenic processes that can also be used for treatment evaluation.

Indexed as

ChoroidEndothelial CellsAnimalsMicePerfusionPlant LectinsRetinal VesselsPlant Lectinstomato lectinchoriocapillarislectinsretinal vasculature

Identifiers

PMID32509436
PMCPMC7255627
OpenAlexW3003747702

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.