ArticleLight, science & applications2020
Symmetrically dispersed spectroscopic single-molecule localization microscopy.
Article in Light, science & applications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 43 citations in OpenAlex.
- Single Fluorogens and Orientation-Localization Microscopy for Quantifying Chemical and Biomolecular Dynamics at the Nanoscale.Accounts of chemical research · 2026Article
- Solvatochromic BODIPYs with Polarity-Independent Absorption for Functional Super-resolution Imaging of Live Cell Membranes.Chemical & biomedical imaging · 2026Article
- SIMBA: an agentic AI platform for single-molecule multidimensional imaging.bioRxiv : the preprint server for biology · 2026Article
- Spectroscopic DNA-PAINT for simultaneous multiplexed super-resolution microscopy.bioRxiv : the preprint server for biology · 2026Article
- Maximizing photon utilization in spectroscopic single-molecule localization microscopy using symmetrically dispersed dual-wedge prisms.Npj imaging · 2026Article
- Automated Cyclic Super-Resolution Microscopy for Nanoscale Protein Mapping.bioRxiv : the preprint server for biology · 2026Article
- Orthogonally Dispersed Spectroscopic Single-Molecule Localization Microscopy.Nanophotonics (Berlin, Germany) · 2026Article
- Framework for Accurate Single-Molecule Spectroscopic Imaging Analyses Using Monte Carlo Simulation and Deep Learning.Analytical chemistry · 2025Article
- DeepQR: single-molecule QR codes for optical gene-expression analysis.Nanophotonics (Berlin, Germany) · 2025Article
- Deep learning-enabled filter-free fluorescence microscope.Science advances · 2025Article
- Multiplexed Nanoscopy via Buffer Exchange.ACS nano · 2024Article
- Deep learning-based spectroscopic single-molecule localization microscopy.Journal of biomedical optics · 2024Article
- Multiscale imaging of corneal endothelium damage and Rho-kinase inhibitor application in mouse models of acute ocular hypertension.Biomedical optics express · 2024Article
- Spectral precision improvement with demagnifying spectral images in spectroscopic nanoscopy.Journal of the Optical Society of America. A, Optics, image science, and vision · 2024Article
- Physically informed Monte Carlo simulation of dual-wedge prism-based spectroscopic single-molecule localization microscopy.Journal of biomedical optics · 2024Article
- Switchable and Functional Fluorophores for Multidimensional Single-Molecule Localization Microscopy.Chemical & biomedical imaging · 2023Review
- Spectroscopic single-molecule localization microscopy: applications and prospective.Nano convergence · 2023Review
- Tetra-color superresolution microscopy based on excitation spectral demixing.Light, science & applications · 2023Article
- Enabling Spectrally Resolved Single-Molecule Localization Microscopy at High Emitter Densities.Nano letters · 2022Article
- Multipair Förster Resonance Energy Transfer via Spectrally Resolved Single-Molecule Detection.The journal of physical chemistry. B · 2022Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Spectroscopic single-molecule localization microscopy (sSMLM) was used to achieve simultaneous imaging and spectral analysis of single molecules for the first time. Current sSMLM fundamentally suffers from a reduced photon budget because the photons from individual stochastic emissions are divided into spatial and spectral channels. Therefore, both spatial localization and spectral analysis only use a portion of the total photons, leading to reduced precisions in both channels. To improve the spatial and spectral precisions, we present symmetrically dispersed sSMLM, or SDsSMLM, to fully utilize all photons from individual stochastic emissions in both spatial and spectral channels. SDsSMLM achieved 10-nm spatial and 0.8-nm spectral precisions at a total photon budget of 1000. Compared with the existing sSMLM using a 1:3 splitting ratio between spatial and spectral channels, SDsSMLM improved the spatial and spectral precisions by 42% and 10%, respectively, under the same photon budget. We also demonstrated multicolour imaging of fixed cells and three-dimensional single-particle tracking using SDsSMLM. SDsSMLM enables more precise spectroscopic single-molecule analysis in broader cell biology and material science applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.