ArticleAmerican journal of translational research2020
SIRT4 suppresses the inflammatory response and oxidative stress in osteoarthritis.
Article in American journal of translational research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Sirtuin 4 Knockout Aggravates Sepsis-Induced Acute Liver Injury by Enhancing Mitochondrial Fission and Mitophagy in Hepatocytes.Mediators of inflammation · 2026Article
- Energy crisis and cartilage collapse: metabolic reprogramming of chondrocytes in osteoarthritis.Frontiers in immunology · 2026Review
- Association between mitochondrial SIRTs (SIRT3, SIRT4, and SIRT5) and PCOS.European journal of medical research · 2025Article
- SIRT4 Protects Müller Glial Cells Against Apoptosis by Mediating Mitochondrial Dynamics and Oxidative Stress.Molecular neurobiology · 2025Article
- Landscape of Histone Posttranslational Modifications in Osteoarthritis.Journal of inflammation research · 2025Review
- Sirtuins and Their Implications in the Physiopathology of Gestational Diabetes Mellitus.Pharmaceuticals (Basel, Switzerland) · 2025Review
- Decreased sirtuin 4 levels promote cellular proliferation and invasion in papillary thyroid carcinoma.European thyroid journal · 2024Article
- Sirtuin 4 (Sirt4) downregulation contributes to chondrocyte senescence and osteoarthritis via mediating mitochondrial dysfunction.International journal of biological sciences · 2024Article
- Silencing KLF6 Alleviates Cigarette Smoke Extract-Induced Mitochondrial Dysfunction in Bronchial Epithelial Cells by SIRT4 Upregulation.International journal of chronic obstructive pulmonary disease · 2024Article
- Metabolic mechanisms orchestrated by Sirtuin family to modulate inflammatory responses.Frontiers in immunology · 2024Review
- Characterizing mitochondrial features in osteoarthritis through integrative multi-omics and machine learning analysis.Frontiers in immunology · 2024Article
- Sirtuin4 alleviates severe acute pancreatitis by regulating HIF-1α/HO-1 mediated ferroptosis.Cell death & disease · 2023Article
- Oxidative stress as a key modulator of cell fate decision in osteoarthritis and osteoporosis: a narrative review.Cellular & molecular biology letters · 2023Review
- A compendium of mitochondrial molecular characteristics provides novel perspectives on the treatment of rheumatoid arthritis patients.Journal of translational medicine · 2023Article
- Improvement of neurological recovery in the insomnia rats by Warming Yang Strategy through targeting SIRT4 by inhibiting inflammation and apoptosis.Immunity, inflammation and disease · 2023Article
- An Integrated View of Stressors as Causative Agents in OA Pathogenesis.Biomolecules · 2023Review
- Epigenetics as a Therapeutic Target in Osteoarthritis.Pharmaceuticals (Basel, Switzerland) · 2023Review
- Role of Sirtuins in the Pathogenesis of Rheumatoid Arthritis.International journal of molecular sciences · 2023Review
- Sirtuins in osteoarthritis: current understanding.Frontiers in immunology · 2023Review
- Natural compounds protect against the pathogenesis of osteoarthritis by mediating the NRF2/ARE signaling.Frontiers in pharmacology · 2023Review
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sirtuins have been involved in the osteoarthritis (OA) process. However, the functions of SIRT4 in the degeneration of human chondrocytes and OA are not fully understood. This study aimed to explore the role of SIRT4 during OA and mechanisms implicated. We extracted total protein and mRNA of the cartilage from OA patients and isolated the chondrocytes from the cartilage in different degenerated degrees for cell culture. Collagen II and SIRT4 levels of the tissues were analyzed by quantitative reverse-transcription polymerase chain reaction (RT-PCR) and Western blot. Chondrocytes were transferred with SIRT4-siRNA, treated with recombinant human SIRT4 protein for 24 h, respectively. Aggrecan, collagen I, collagen II, MMP-13, IL-6, TNF-α, SOD1, SOD2, and CAT expression, and ROS levels were investigated by Western blot, RT-PCR, immunofluorescence, enzyme-linked immunosorbent assay (ELISA), or flow cytometry. Collagen II decreased significantly in severely degenerated cartilage compared to the mild one, paralleling with SIRT4 expression both in protein and mRNA levels. Chondrocytes in severe OA grade were observed with a decrease in aggrecan, collagen II, SOD1, SOD2, CAT expression, nonetheless, an increase in collagen I, reactive oxygen species (ROS), MMP-13, IL-6, and TNF-α levels. However, SRIT4 protein treatment significantly upregulated aggrecan, collagen II, an antioxidant enzyme, and suppressed ROS and inflammatory response. Further analysis revealed that silencing of SIRT4 expression induced healthy chondrocytes, a decrease in aggrecan, collagen II and antioxidant enzyme expression, and an increase in ROS and inflammatory response, importantly, which can be reversed by SIRT4 protein stimuli. Our results elucidated that SIRT4 was tangled with the development of OA, and SIRT4 overexpression contributes to suppresses the inflammatory response and oxidative stress.
Indexed as
Identifiers
32509191PMC7270038What OpenQuestion holds
Registered trials
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