ArticleNature communications2020
Spitzenkörper assembly mechanisms reveal conserved features of fungal and metazoan polarity scaffolds.
Article in Nature communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 32 citations in OpenAlex.
- A short linear motif, conserved from yeast to human, binds to members of the Spa2 family of cortical scaffold proteins.Journal of cell science · 2026Article
- Oligomerization and exocyst coupling underlie Spa2-mediated focusing of polarized growth in fission yeast.Journal of cell science · 2025Article
- The phase-separating Magnaporthe oryzae MoSpa2 complex organizes actin nucleation centers for plant infection.The Plant cell · 2025Article
- The Exocyst Subunits EqSec5 and EqSec6 Promote Powdery Mildew Fungus Growth and Pathogenicity.Journal of fungi (Basel, Switzerland) · 2025Article
- The mRNA stability factor Khd4 defines a specific mRNA regulon for membrane trafficking in the pathogenProceedings of the National Academy of Sciences of the United States of America · 2023Article
- The type V myosin-containing complex HUM is a RAB11 effector powering movement of secretory vesicles.iScience · 2022Article
- The cellular slime mold Fonticula alba forms a dynamic, multicellular collective while feeding on bacteria.Current biology : CB · 2022Article
- A teamwork promotion of formin-mediated actin nucleation by Bud6 and Aip5 inMolecular biology of the cell · 2022Article
- Crosstalk between basal extracellular matrix adhesion and building of apical architecture during morphogenesis.Biology open · 2021Review
- Article
- Tracking Fungal Growth: Establishment of Arp1 as a Marker for Polarity Establishment and Active Hyphal Growth in Filamentous Ascomycetes.Journal of fungi (Basel, Switzerland) · 2021Article
- Turning Inside Out: Filamentous Fungal Secretion and Its Applications in Biotechnology, Agriculture, and the Clinic.Journal of fungi (Basel, Switzerland) · 2021Review
- Article
- The GUL-1 Protein Binds Multiple RNAs Involved in Cell Wall Remodeling and Affects the MAK-1 Pathway inFrontiers in fungal biology · 2021Article
- Orchestrated actin nucleation by theThe Journal of biological chemistry · 2020Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Spitzenkörper (SPK) constitutes a collection of secretory vesicles and polarity-related proteins intimately associated with polarized growth of fungal hyphae. Many SPK-localized proteins are known, but their assembly and dynamics remain poorly understood. Here, we identify protein-protein interaction cascades leading to assembly of two SPK scaffolds and recruitment of diverse effectors in Neurospora crassa. Both scaffolds are transported to the SPK by the myosin V motor (MYO-5), with the coiled-coil protein SPZ-1 acting as cargo adaptor. Neither scaffold appears to be required for accumulation of SPK secretory vesicles. One scaffold consists of Leashin-2 (LAH-2), which is required for SPK localization of the signalling kinase COT-1 and the glycolysis enzyme GPI-1. The other scaffold comprises a complex of Janus-1 (JNS-1) and the polarisome protein SPA-2. Via its Spa homology domain (SHD), SPA-2 recruits a calponin domain-containing F-actin effector (CCP-1). The SHD NMR structure reveals a conserved surface groove required for effector binding. Similarities between SPA-2/JNS-1 and the metazoan GIT/PIX complex identify foundational features of the cell polarity apparatus that predate the fungal-metazoan divergence.
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