Evidence map›Paper›PMID 32503264›Full record

ArticleBiology2020

Hydralazine Sensitizes to the Antifibrotic Effect of 5-Aza-2'-deoxycytidine in Hepatic Stellate Cells.

Kiyoshi Asada, Kosuke Kaji, Shinya Sato, Kenichiro Seki, Naotaka Shimozato, Hideto Kawaratani, Hiroaki Takaya, Yasuhiko Sawada, Keisuke Nakanishi, Masanori Furukawa and 6 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 45% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors at 1 institution in 1 country.

Kiyoshi AsadaThird Department of Internal Medicine, Nara Medical University, Kashihara, Nara 634-8521, Japan.
Kosuke KajiThird Department of Internal Medicine, Nara Medical University, Kashihara, Nara 634-8521, Japan.
Shinya SatoThird Department of Internal Medicine, Nara Medical University, Kashihara, Nara 634-8521, Japan.ORCID 0000-0003-3049-3443
Kenichiro SekiThird Department of Internal Medicine, Nara Medical University, Kashihara, Nara 634-8521, Japan.
Naotaka ShimozatoThird Department of Internal Medicine, Nara Medical University, Kashihara, Nara 634-8521, Japan.
Hideto KawarataniThird Department of Internal Medicine, Nara Medical University, Kashihara, Nara 634-8521, Japan.ORCID 0000-0002-4361-0592
Hiroaki TakayaThird Department of Internal Medicine, Nara Medical University, Kashihara, Nara 634-8521, Japan.
Yasuhiko SawadaThird Department of Internal Medicine, Nara Medical University, Kashihara, Nara 634-8521, Japan.
Keisuke NakanishiThird Department of Internal Medicine, Nara Medical University, Kashihara, Nara 634-8521, Japan.
Masanori FurukawaThird Department of Internal Medicine, Nara Medical University, Kashihara, Nara 634-8521, Japan.
Mitsuteru KitadeThird Department of Internal Medicine, Nara Medical University, Kashihara, Nara 634-8521, Japan.
Kei MoriyaThird Department of Internal Medicine, Nara Medical University, Kashihara, Nara 634-8521, Japan.
Tadashi NamisakiThird Department of Internal Medicine, Nara Medical University, Kashihara, Nara 634-8521, Japan.ORCID 0000-0002-3158-5318
Ryuichi NoguchiThird Department of Internal Medicine, Nara Medical University, Kashihara, Nara 634-8521, Japan.
Takemi AkahaneThird Department of Internal Medicine, Nara Medical University, Kashihara, Nara 634-8521, Japan.ORCID 0000-0002-6675-0475
Hitoshi YoshijiThird Department of Internal Medicine, Nara Medical University, Kashihara, Nara 634-8521, Japan.
Nara Medical University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundHepatic stellate cell (HSC) activation is essential for the development of liver fibrosis. Epigenetic machinery, such as DNA methylation, is largely involved in the regulation of gene expression during HSC activation. Although the pharmacological DNA demethylation of HSC using 5-aza-2'-deoxycytidine (5-aza-dC) yielded an antifibrotic effect, this drug has been reported to induce excessive cytotoxicity at a high dose. Hydralazine (HDZ), an antihypertensive agent, also exhibits non-nucleoside demethylating activity. However, the effect of HDZ on HSC activation remains unclear. In this study, we performed a combined treatment with 5-aza-dC and HDZ to obtain an enhanced antifibrotic effect with lower cytotoxicity.

methodsHSC-T6 cells were used as a rat HSC cell line in this study. The cells were cultivated together with 1 µM 5-Aza-dC and/or 10 µg/mL of HDZ, which were refreshed every 24 h until the 96 h treatment ended. Cell proliferation was measured using the WST-1 assay. The mRNA expression levels of peptidylprolyl isomerase A (

resultsThe percentage cell viability with 5-aza-dC, HDZ, and combined treatment vs. the vehicle-only control was 101.4 ± 2.5, 95.2 ± 5.7, and 79.2 ± 0.7 (

conclusionsFurther confirmation using primary HSCs and in vivo animal models is desirable, but combined treatment with 5-aza-dC and HDZ may be an effective therapy for liver fibrosis without severe adverse effects.

Indexed as

5-aza-2′-deoxycytidineDNA methylationhepatic stellate cellhydralazineliver fibrosis

Identifiers

PMID32503264
PMCPMC7345531
OpenAlexW3034056409

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.