Evidence map›Paper›PMID 32502464›Full record

ArticleStem cell reports2020

Cryopreserved Human Oocytes and Cord Blood Cells Can Produce Somatic Cell Nuclear Transfer-Derived Pluripotent Stem Cells with a Homozygous HLA Type.

Jeoung Eun Lee, Ji Yoon Lee, Chang-Hwan Park, Jin Hee Eum, Soo Kyung Jung, A-Reum Han, Dong-Won Seol, Jin Saem Lee, Hyun Soo Shin, Jung Ho Im and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Jeoung Eun LeeCHA Advanced Research Institute, CHA University, Seongnam, Gyunggi-do 13488, Korea.
Ji Yoon LeeCHA Advanced Research Institute, CHA University, Seongnam, Gyunggi-do 13488, Korea.
Chang-Hwan ParkGraduated School of Biomedical Science and Engineering, Hanyang University, Seoul 04763, Korea.
Jin Hee EumFertility Center of CHA Gangnam Medical Center, CHA University, Seoul 06135, Korea.
Soo Kyung JungCHA Advanced Research Institute, CHA University, Seongnam, Gyunggi-do 13488, Korea.
A-Reum HanDepartment of Biomedical Science, CHA University, Seongnam, Gyunggi-do 13488, Korea.
Dong-Won SeolDepartment of Biomedical Science, CHA University, Seongnam, Gyunggi-do 13488, Korea.
Jin Saem LeeGraduated School of Biomedical Science and Engineering, Hanyang University, Seoul 04763, Korea.
Hyun Soo ShinDepartment of Radiation Oncology, CHA Bundang Medical Center, CHA University, Seongnam 13496, Korea.
Jung Ho ImDepartment of Radiation Oncology, CHA Bundang Medical Center, CHA University, Seongnam 13496, Korea.
Taehoon ChunDepartment of Biotechnology, College of Life and Biotechnology, Korea University, Seoul 02841, Korea.
Kyungsoo HaNew Drug Development Center, Osong Medical Innovation Foundation, Osong 28160, Korea.
Deok Rim HeoNew Drug Development Center, Osong Medical Innovation Foundation, Osong 28160, Korea.
Tae Ki YoonDepartment of Biomedical Science, CHA University, Seongnam, Gyunggi-do 13488, Korea.
Dong Ryul LeeCHA Advanced Research Institute, CHA University, Seongnam, Gyunggi-do 13488, Korea; Department of Biomedical Science, CHA University, Seongnam, Gyunggi-do 13488, Korea. Electronic address: drleedr@cha.ac.kr.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Human pluripotent stem cells (PSCs) through somatic cell nuclear transfer (SCNT) may be an important source for regenerative medicine. The low derivation efficiency of stem cells and the accessibility of human oocytes are the main obstacles to their application. We previously reported that the efficiency of SCNT was increased by overexpression of H3K9me3 demethylase. Here, we applied a modified derivation method to the PSC line and first obtained human SCNT-PSC lines derived from both donated cryopreserved oocytes and cord blood cells with a homozygous human leukocyte antigen (HLA) type. The SCNT-PSCs have very similar characteristics with embryonic stem cells (ESCs) and additionally have shown immunocompatibility in an in vitro and in vivo humanized mouse with a matching HLA type. Our study demonstrates that SCNT technology using donated cryopreserved oocytes and cord blood cells with a known HLA type provides a promising method for establishing a human HLA-matched SCNT-PSC bank for regenerative medicine.

Indexed as

CryopreservationNuclear Transfer TechniquesAnimalsBiomarkersCell DifferentiationCell LineCell LineageFetal BloodHLA AntigensHomozygoteHumansMiceModels, AnimalOocytesOsteoblastsPluripotent Stem CellsBiomarkersHLA Antigensfrozen cord blood cellsfrozen oocyteshomozygous HLApluripotent stem cellssomatic cell nuclear transfer

Identifiers

PMID32502464
PMCPMC7363744

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.